文献类型：期刊文献

英文题名：Binary System for MicroRNA-Targeted Imaging in Single Cells and Photothermal Cancer Therapy

作者：Qian, Ruo-Can[1];Cao, Yue[1];Long, Yi-Tao[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2016

卷号：88

期号：17

起止页码：8640

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20163702792965);WOS:【SCI-EXPANDED(收录号:WOS:000382805900043)】；

基金：This research was supported by the National Natural Science Foundation of China (21327807), the Science Fund for Creative Research Groups (21421004), the Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning (YJ0130.504), the Program of Shanghai Subject Chief Scientist (15XD1501200), the Programme of Introducing Talents of Discipline to Universities (B16017), the China Postdoctoral Science Foundation (2015M570335, 2016T90339), and the State Key Laboratory of Analytical Chemistry for Life Science Open Foundation (SKLACLS1512).

语种：英文

外文关键词：Cytology - Cells - Coremaking - RNA - Gold nanoparticles - Diseases - Liposomes - Cancer cells - Infrared devices

摘要：Abnormal expression of microRNAs (miRNAs) is often associated with tumorigenesis, metastasis, and progression. Among them, miRNA-21 is found to be overexpressed in most of the cancer cells. Here, a binary system is designed for miRNA-21 targeted imaging and photothermal treatment in single cells. The binary system is composed by a pair of probes (probe-1 and probe-2), which are encapsulated in liposomes for cell delivery. Both of the two probes adopt gold nanoparticles (AuNPs) as the core material, and the AuNPs are functionalized with Cy5-marked molecular beacon (MB-1/MB-2 for probe-1/probe-2, respectively). The loop part of MBs are designed to be complementary with miRNA-21. Therefore, after the binary system enters into the cytoplasm, MBs can be opened upon miRNA 21 triggered hybridization, which turns "on" the fluorescence of Cy5 of the localization of miRNA-21. At the same time, a cross-linking between the probes occurs since the far ends of MB-1 and MB-2 are designed to be complementary with each other. The miRNA-induced aggregation shifts the absorption of AuNPs to near infrared, which can be observed under dark-field microscopy (DFM) and used for the following photothermal therapy. Under near-infrared (NIR) irradiation, MCF-7 breast cancer cells are successfully killed. The proposed system can be further applied in tumor-bearing mice and shows significant therapeutic effect. This work provides a new tool for intracellular miRNA analysis and targeted treatment against cancer.