文献类型：期刊文献

中文题名：转小鼠金属硫蛋白基因集胞藻6803及其野生藻培养

英文题名：Preliminary Studies on the Culture of Transgenic Synechocystis sp. PCC6803 with Mouse Metallothionein Gene and Synechocystis sp. PCC6803

作者：王淑璠[1];李元广[1];李志勇[1];施定基[2];茹炳根[3];张嗣良[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237;[2]中国科学院植物研究所,北京100093;[3]北京大学生命科学学院,北京100871

年份：2000

卷号：26

期号：1

起止页码：48

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;EI(收录号：2000095313543);Scopus;北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；

基金：国家"九五"科技攻关项目!(96-C02-04-05);海洋生物技术863 项目!(819-Q-01)资助

语种：中文

中文关键词：转基因集胞藻;光合自养;金属硫蛋白;蓝藻

外文关键词：transgenic Synechocystis sp. PCC6803; Synechocystis sp. PCC6803; photoautotrophy; metallothionein

摘要：通过摇瓶培养考察转小鼠金属硫蛋白基因集胞藻６８０３（转基因藻）及其野生藻（集胞藻６８０３）的光合自养培养过程中碳源（碳酸钠）浓度、氮源（硝酸钠）浓度和光照度对这两种藻生长的影响。野生藻的最适生长碳源浓度（０．０５～０．１ｇ／Ｌ）比转基因藻的最适生长碳源浓度（０．０２～０．０５ｇ／Ｌ）高。氮源浓度对转基因藻和野生藻的影响趋势相同。在０．３～４．５ｇ／Ｌ范围内，随着氮源浓度的增高，藻体生长的最大细胞浓度降低。转基因藻生长的最佳光照度为１ ５００ｌｘ，野生藻生长的最佳光照度为３
The influences of Na 2CO 3 concentration, NaNO 3 concentration and light intensity on the photoautotrophic growth of transgenic Synechocystis sp PCC6803 with mouse metallothionein gene and Synechocystis sp. PCC6803 in the flask are studied. The optimum Na 2CO 3 concentration of Synechocystis sp. PCC6803 is 0.05～0.1g/L, which is higher than that (0.02～0.05g/L) of transgenic Synechocystis sp. PCC6803. During the range of 0.3～4.5g/L, the effects of NaNO 3 concentration in the medium on the cell growth of Synechocystis sp. PCC6803 are similar to that of transgenic Synechocystis sp PCC6803, the higher the NaNO 3 concentration, the lower the maximum cell concentration. The optimum light intensity for the growth of transgenic Synechocystis sp. PCC6803 is 1 500lx, while that of Synechocystis sp. PCC6803 is 3 000lx.