文献类型：期刊文献

英文题名：Central pathway engineering for enhanced succinate biosynthesis from acetate in Escherichia coli

作者：Huang, Bing[1];Yang, Hao[1];Fang, Guochen[1];Zhang, Xing[1];Wu, Hui[1,2,3];Li, Zhimin[1,2];Ye, Qin[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai, Peoples R China;[3]China Natl Light Ind Council, Key Lab Biobased Mat Engn, Shanghai, Peoples R China

年份：2018

卷号：115

期号：4

起止页码：943

外文期刊名：BIOTECHNOLOGY AND BIOENGINEERING

收录：;EI(收录号：20180404673406);WOS:【SCI-EXPANDED(收录号:WOS:000426493300013)】；

基金：National Natural Science Foundation of China, Grant number: 21776083; National Science Foundation for Young Scientist of China, Grant number: 21406065; Science and Technology Commission of Shanghai Municipality, Grant number: 17JC1404800; National Key Research and Development Program of China, Grant number: 2017YFB0039302; Foundation of Key Laboratory for Industrial Biocatalysis (Tsinghua University), Ministry of Education, Grant number: 2015101

语种：英文

外文关键词：acetate; cofactor engineering; Escherichia coli; metabolic engineering; succinate

摘要：Acetate, a non-food based substrate obtained from multiple biological and chemical ways, is now being paid great attention in bio-manufacturing and have a strong potential to compete with sugar-based carbon source. In this study, acetate can be efficiently converted to succinate by engineered Escherichia coli strains via the combination of several metabolic engineering strategies, including reducing OAA decarboxylation, engineering TCA cycle, enhancement of acetate assimilation pathway and increasing aerobic ATP supply through cofactor engineering. The engineered strain HB03(pTrc99a-gltA, pBAD33-Trc-fdh) accumulated 30.9mM of succinate in 72hr and the yield reached the maximum theoretical yield (similar to 0.50mol/mol). In the resting-cell experiments, the yield of succinate in HB03(pTrc99a-gltA) and HB03(pTrc99a-gltA, pBAD33-Trc-fdh) dropped dramatically, although the productivity of succinate increased due to the high cell density. Further deletion of icdA, formed HB04(pTrc99a-gltA) and HB04(pTrc99a-gltA, pBAD33-Trc-fdh), increased the yield of succinate in the resting-cell experiments. The highest concentration of succinate achieved 194mM and the yield reached 0.44mol/mol in 16hr by HB04(pTrc99a-gltA, pBAD33-Trc-fdh). The results showed the metabolically engineered E. coli strains have great potential to produce succinate from acetate.