文献类型：期刊文献

中文题名：葡萄糖和Cu^(2+)对蛹虫草Cu，Zn超氧化物歧化酶在E．coli中表达的影响

英文题名：Effect of Glucose and Cu^(2+) on the Expression of Cordyceps militaris Cu,Zn-Superoxide Dismutase in E.coli

作者：王尊生[1];何卓晶[1];宋聿文[1];袁勤生[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室

年份：2006

卷号：37

期号：8

起止页码：522

中文期刊名：中国医药工业杂志

外文期刊名：Chinese Journal of Pharmaceuticals

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：Cu,Zn-超氧化物歧化酶;蛹虫草;表达

外文关键词：Cu, Zn-superoxide dismutase; Cordyceps militaris; expression

摘要：对含有蛹虫草Cu,Zn-超氧化物歧化酶(cm-SOD)基因的重组E．coli培养条件进行了初步优化。结果表明,LB培养基国含有Cu^(2+)可明显提高超氧化物歧化酶比活力；而在菌体培养中补加葡萄糖,可提高重组蛋白表达量和细胞生物量。在含有0．6mmol/L Cu^(2+)、Zn^(2+)的LB培养基中补加22mmol/L葡萄糖,每200ml培养液可获得lg湿细胞,超氧化物歧化酶比活力达到3305u/mg。
The culture conditions for expression of Cordyceps militaris Cu, Zn-superoxide dismutase in E.coli were optimized. The results showed that adding Cu^2＋in culture medium could increase the specific activity of superoxide dismutase in E.coli, whereas supplement with glucose during shake flask incubation could increase the amount of biomass and recombinant protein. One gram of wet cell weight could be obtained from 200ml culture when LB medium cantaining 0.6mmol（L Cu^2＋ and Zn^2＋ and supplementing with 22mmol/L glucose was used, and specific activity of superoxide dismutase was 3305u/mg.