文献类型：期刊文献

英文题名：Microbial community dynamics in Baolige oilfield during MEOR treatment, revealed by Illumina MiSeq sequencing

作者：You, Jing[1,3];Wu, Gang[1];Ren, Fuping[1];Chang, Qi[2];Yu, Bo[2];Xue, Yanfen[2];Mu, Bozhong[3]

机构：[1]Huabei Oilfield Ltd, Petr Prod Engn Inst, Renqiu 062552, Peoples R China;[2]Chinese Acad Sci, Inst Microbiol, Beijing 100101, Peoples R China;[3]E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2016

卷号：100

期号：3

起止页码：1469

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;EI(收录号：20154401458838);WOS:【SCI-EXPANDED(收录号:WOS:000369308900036)】；

基金：This work was funded by grants from Huabei Oilfield Ltd. (Grant No. 2014E-3507) and the National Natural Science Foundation of China (31170116).

语种：英文

外文关键词：MEOR-treated oil reservoir; High-throughput sequencing; Microbial community; Microbial community dynamics

摘要：This study was carried out to understand microbial diversity and function in the microbial enhanced oil recovery (MEOR) process and to assess the impact of MEOR treatment on the microbial community in an oil reservoir. The Illumina MiSeq-based method was used to investigate the structure and dynamics of the microbial community in a MEOR-treated block of the Baolige oilfield, China. The results showed that microbial diversity was high and that 23 phyla occurred in the analyzed samples. Proteobacteria, Firmicutes, Bacteroidetes, Thermotogae, and Euryarchaeota were present in relatively high abundance in all analyzed samples. Injection of bacteria and nutrients resulted in interesting changes in the composition of the microbial community. During MEOR treatment, the community was dominated by the known hydrocarbon-utilizing genera Pseudomonas and Acinetobacter. After the treatment, the two genera decreased in abundance over time while Methanobacteriaceae, as well as known syntrophic genera such as Syntrophomonas, Pelotomaculum, Desulfotomaculum, and Thermacetogenium gradually increased. The change in dominant microbial populations indicated the presence of a succession of microbial communities over time, and the hydrocarbon degradation and syntrophic oxidation of acetate and propionate to methane in the MEOR-treated oilfield. This work contributes to a better understanding of microbial processes in oil reservoirs and helps to optimize MEOR technology.