文献类型：期刊文献

英文题名：Difenoconazole induces oxidative DNA damage and mitochondria mediated apoptosis in SH-SY5Y cells

作者：Wang, Xin[1];Ni, Hongfei[1];Xu, Wenping[1];Wu, Bing[1];Xie, Te[1];Zhang, Cheng[2];Cheng, Jiagao[1];Li, Zhong[1];Tao, Liming[1];Zhang, Yang[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Chem Biol, Sch Pharm, Shanghai 200237, Peoples R China;[2]UT Southwestern Med Ctr, Dept Pathol, Dallas, TX 75390 USA

年份：2021

卷号：283

外文期刊名：CHEMOSPHERE

收录：;EI(收录号：20212510518424);WOS:【SCI-EXPANDED(收录号:WOS:000692108200008)】；

基金：This work was financial Sponsored by Shanghai Sailing Program (NO.19YF1411100) and Innovation Program of Shanghai Municipal Education Commission, China (NO. 201701070002E00037) .

语种：英文

外文关键词：Difenoconazole; Cytotoxicity; DNA damage; Apoptosis; Human neuroblastoma SH-SY5Y cells

摘要：Difenoconazole is one of the most typical triazole fungicides. Difenoconazole is widely used in the field of agricultural production, and its health and safety problems need to be further studied. The main purpose of this paper is to verify the neurotoxicity of Difenoconazole at the cellular level. In this study, SH-SY5Y cell line of human neuroblastoma was used to evaluate its potentially toxic effects and molecular mechanism in vitro. The research indicated that Difenoconazole could reduce cell viability and inhibit cell proliferation, induce DNA damage and accelerate programmed cell death. Further studies showed that Difenoconazole induced DNA double-strand breaks, intracellular generation of ROS, cleaved PARP, mitochondrial membrane potential collapse, induced Cyt c release, and Bax/Bcl-2 ratio increase in SH-SY5Y cells. In conclusion, the cytotoxicity of Difenoconazole revealed its toxic effect on SH-SY5Y cells, and the IC50 value was 55.41 mu M after 24 h exposure. Meanwhile, the genetic toxicity of Difenoconazole has revealed that it can induce DNA damage and apoptosis of SH-SY5Y cells. Through this study, the toxic effects of Difenoconazole on SH-SY5Y cells are further understood, which provides a more scientific basis for its safe use and risk control.