文献类型：期刊文献

中文题名：基于三(2-氨基乙基)胺和6-羧基荧光素的双模式核磁共振成像造影剂

英文题名：Synthesis of Bimodal Magnetic Resonance Imaging Contrast Agent from Tris(2-aminoethyl)amine and 6-Carboxy-Fluorescein

作者：潘斐[1];朱勤[1];钱伟桥[1];胡爱国[1]

机构：[1]华东理工大学材料科学与工程学院,上海市先进聚合物材料重点实验室,上海200237

年份：2016

卷号：42

期号：6

起止页码：758

中文期刊名：华东理工大学学报(自然科学版)

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：国家自然科学基金(21274042)

语种：中文

中文关键词：核磁共振成像;造影剂;三(2-氨基乙基)胺;荧光成像;量子产率

外文关键词：magnetic resonance imaging;contrast agent;TREN;fluorescence imaging;quantum yield

摘要：通过将三(2-氨基乙基)胺(TREN)、6-羧基荧光素与溴乙酸苄酯结合而合成出多胺多羧型配体,并赋予化合物荧光成像的功能。进一步与顺磁性的六水合氯化钆(Ⅲ)反应获得兼具核磁共振成像(Magnetic Resonance Imaging,MRI)和光学成像功能的双模式核磁共振成像造影剂。通过核磁氢谱、碳谱、高分辨质谱和ICP-AES表征了配体和金属配合物的结构。研究结果表明,该配合物的纵向弛豫率(r_1)为3.46mM^(-1)·s^(-1),与小分子型商用造影剂如马根维显~(Gd-DTPA)接近。该配合物同时拥有66%的荧光量子产率,在紫外光照射下显示明亮的绿色荧光,为以后进行生物体内研究打下坚实基础。
A fluorescent ligand with polydentate amino carboxylate moieties was synthesized from tris(2-aminoethyl)amine and benzyl 2-bromoacetate,followed by the introduction of fluorescent functionality through an amidation reaction between the primary amine group and 6-carboxy-fluorescein.After coordination with paramagnetic GdCl3·6H2O,a bimodal contrast agent combing the ability for magnetic resonance imaging(MRI)and optical imaging was formed.The structures of the ligands and the complexes were confirmed by both H-and C-NMR.high resolution mass spectrometry,and ICP-AES.This new MRI contrast agent showed a r1 value(longitudinal relaxivity)of 3.46 mM^-1·s^-1,comparable to commercial small molecular contrast agent like Magevist(Gd-DTPA).This complex also showed bright green fluorescence when shined with UV light,showing a high quantum yield of 66%,which is essential for clinical bimodal imaging.