文献类型：期刊文献

英文题名：Tolerance potential of Chilo suppressalis larvae to fipronil exposure via the modulation of detoxification and GABA responses

作者：Xiao, Ciying[3];Luan, Shaorong[2];Xu, Zhikang[1];Lang, Jialin[1];Rao, Wenbing[1];Huang, Qingchun[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Res Ctr Anal & Test, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2017

卷号：20

期号：4

起止页码：1287

外文期刊名：JOURNAL OF ASIA-PACIFIC ENTOMOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000419749300033)】；

基金：This work was financially supported by the Shanghai Foundation of Science and Technology (No. 15431906800).

语种：英文

外文关键词：Chilo suppressalis; Fipronil; Detoxification enzymes; Glutamate; Gamma-aminobutyric acid

摘要：The Asiatic rice borer, Chilo suppressalis Walker, is one of the most destructive rice pests in the world; its physiological adaptability to the sustained application of fipronil was investigated. After topical application, fipronil showed strong lethal effect with LD20 and LD80 values on the fourth instar larvae as 0.00159 and 0.00906 mu g per larvae at 48 h- post treatment, and also revealed slight inhibition on the eclosion of pupae and the hatching of eggs. LD20 dosage of fipronil significantly enhanced the activities of carboxylesterase (CarE), glutathione-S-transferase (GST) and cytochrome P450-linked p-nitro O-demethylase (PNOD) in the borers. LD80 dosage of fipronil showed a mild decrease on the activity of CarE, GST and PNOD, and a slight suppression on the activity of glutamate dehydrogenase (GDH), but strongly induced the transform of glutamate to gamma-aminobutyric acid (GABA) and the activity of glutamate decarboxylase (GAD), thereby improved GABA level in the bores. Results suggested that the borer possessed positive tolerance potential to firponil via the modulation of detoxification and GABA responses and provided the basis for further studies on reducing the tolerance of the borer in the control strategies.