文献类型：会议论文

英文题名：Synthesis of a macroporous hydrophilic ternary copolymer and its application in boronate-affinity separation

作者：Lei, Yinlin[1]; Liu, Zuozhen[1]; Liu, Qinfeng[1]; Wu, Xingyan[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

会议论文集：9th International Conference on Polymer-Based Technology (POC 2000)

会议日期：MAY 21-26, 2000

会议地点：NANKAI UNIV, TIANJIN, PEOPLES R CHINA

主办单位：NANKAI UNIV

语种：英文

外文关键词：vinyl acetate-glycidyl methacrylate-allyl methacrylate copolymer; suspension polymerization; boronate-affinity separation; polysaccharide peptide of Coriolus versicolor

摘要：A new macroporous ternary copolymer was synthesized using vinyl acetate (VAC), glycidyl methacrylate (GMA) and allyl methacrylate (AMA) through suspension polymerization with a mixture of n-heptane and butyl acetate as the porogenic agent. The effects of the crosslinking degree, the level of GMA and the porogenic agent mixture and composition on the pore structure of the copolymer and on the properties of the alcoholysed copolymer were investigated. The properties of a typical adsorbent were pore diameter 18.9 nm, pore volume 0.38 ml/g, and specific surface area 80.2 m(2)/g. A hydrophilic polyvinyl alcohol-based matrix was obtained on alcoholysis of the copolymer with the epoxy group unaffected. The alcoholysed copolymer was then attached to a spacer, 6-aminocaproic acid (6-ACA), and finally coupled to 3-aminophenylboronic acid (APBA) as a ligand. The addition reaction between the epoxy group of the matrix and the amino group of 6-ACA was also examined. The ligand density of the prepared affinity adsorbent was 0.865 mmol/g dry resin, which was applied to purify a polysaccharide peptide of Coriolus versicolor (PSP). The optimal conditions for the adsorption of PSP was 0.2 M ammonium acetate (pH 8.2) containing 0.2 M NaCl. (C) 2001 Published by Elsevier Science B.V.