文献类型：期刊文献

中文题名：青稞苗二十六烷醇的提取优化及其预防HepG2细胞脂质过氧化研究

英文题名：Preventive effects of hexacosanol from barley seedlings on lipid peroxidation in HepG2 cells

作者：黎穗子[1];胥孙婕[1];曹承嘉[1];常雅宁[1]

机构：[1]华东理工大学生物工程学院,上海200237

年份：2019

卷号：44

期号：1

起止页码：263

中文期刊名：食品科技

外文期刊名：Food Science and Technology

收录：北大核心:【北大核心2017】；

语种：中文

中文关键词：二十六烷醇;提取优化;棕榈酸;HepG2细胞;脂质过氧化

外文关键词：hexcosanol;extraction optimization;palmitic acid;HepG2 cells;lipid peroxidation

摘要：目的:优化青稞苗二十六烷醇提取工艺,研究它对棕榈酸(Palmitic acid,PA)诱导的Hep G2细胞脂质过氧化预防作用。方法:以气相色谱(GC)测定二十六烷醇提取量为指标,通过单因素和正交试验优化提取工艺,MTT法检测细胞活力,采用PA诱导、油红O染色和活性氧(ROS)测定建立Hep G2细胞脂质过氧化模型,微板法测定细胞甘油三酯(Triglyceride,TG)、超氧化物歧化酶(Superoxide dismutase, SOD)、谷丙转氨酶(Alanine aminotransferase,ALT)和谷草转氨酶(Aspartate aminotransferase,AST)水平。结果:最优提取工艺为料液比1:10 g/mL、提取温度85℃、8%氢氧化钠乙醇溶液、提取时间3.0 h,二十六烷醇预处理能预防Hep G2细胞造模后TG、ALT、AST升高和SOD降低。结论:测得青稞苗二十六烷醇提取率为(330±60)mg/100 g,其能有效预防Hep G2细胞的脂质过氧化损伤。
Objective: To optimize the extraction process of hexacosanol from barley seedlings and study its preventive effects on palmitic acid(PA)-induced lipid peroxidation in HepG2 cells. Methods: The extraction amount of hexacosanol was determined by gas chromatography(GC). The extraction process was optimized by single factor and orthogonal test. Cell viability was detected by MTT assay. PA induction, Oil red O staining and reactive oxygen species(ROS) were used. The lipid peroxidation model of HepG2 cells was established and the cell triglyceride(TG), superoxide dismutase(SOD), alanine aminotransferase(ALT) and aspartate aminotransferase(AST) level were determined by microplate method. Results: The optimum extraction process were as follows: solid-liquid ratio of 1:10 g/mL, extraction temperature 85 ℃, 8% sodium hydroxide in ethanol as solvent, extraction time 3.0 h. Pretreatment with hexacosanol can prevent TG, ALT, AST increases and SOD decrease in model cells. Conclusion: After optimization process, the content of hexacosanol from barley seedlings was(330±60)mg/100 g, which could effectively prevent the lipid peroxidation damage of HepG2 cells.