文献类型：期刊文献

英文题名：Genome-Wide Identification of Fitness Factors in Seawater for Edwardsiella piscicida

作者：Wei, Lifan[1];Wu, Yanyan[1];Yang, Guanhua[1];Xu, Rongjing[4];Liu, Xiaohong[1,2,3];Liu, Qin[1,2,3];Zhang, Yuanxing[1,2,3];Ma, Yue[1,2,3];Wang, Qiyao[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg, Shanghai, Peoples R China;[4]Yantai Tianyuan Aquat Co Ltd, Yantai, Shandong, Peoples R China

年份：2019

卷号：85

期号：10

外文期刊名：APPLIED AND ENVIRONMENTAL MICROBIOLOGY

收录：;EI(收录号：20192006930799);WOS:【SCI-EXPANDED(收录号:WOS:000466715500010)】；

基金：This study was supported by grants from the National Natural Science Foundation of China (31602200 to X.L. and 31430090 to Y.Z.), the Ministry of Agriculture of China (CARS-47), and the Science and Technology Commission of Shandong and Shanghai Municipality (2017CXGC0103 and 17391902000).

语种：英文

外文关键词：Edwardsiella piscicida; T3SS; transposon insertion sequencing; TIS; oxidative phosphorylation; seawater; temperature

摘要：Marine pathogens are transmitted from one host to another through seawater. Therefore, it is important for marine pathogens to maintain survival or growth in seawater. However, little is known about how marine pathogens adapt to living in seawater environments. Here, transposon insertion sequencing was performed to explore the genetic determinants of Edwardsiella piscicida survival in seawater at 16 and 28 degrees C. Seventy-one mutants with mutations mainly in metabolism-, transportation-, and type III secretion system (T3SS)-related genes showed significantly increased or impaired fitness in 16 degrees C water. In 28 degrees C seawater, 63 genes associated with transcription and translation, as well as energy production and conversion, were essential for optimal survival of the bacterium. In particular, 11 T3SS-linked mutants displayed enhanced fitness in 16 degrees C seawater but not in 28 degrees C seawater. In addition, 13 genes associated with oxidative phosphorylation and 4 genes related to ubiquinone synthesis were identified for survival in 28 degrees C seawater but not in 16 degrees C seawater, which suggests that electron transmission and energy-producing aerobic respiration chain factors are indispensable for E. piscicida to maintain survival in higher-temperature seawater. In conclusion, we defined genes and processes related to metabolism and virulence that operate in E. piscicida to facilitate survival in low- and high-temperature seawater, which may underlie the infection outbreak mechanisms of E. piscicida and facilitate the development of improved vaccines against marine pathogens. IMPORTANCE Edwardsiella piscicida is one of the most important marine pathogens and causes serious edwardsiellosis in farmed fish during the summer-autumn seasonal changes, resulting in enormous losses to aquaculture industries worldwide. Survival and transmission of the pathogen in seawater are critical steps that increase the risk of outbreaks. To investigate the mechanism of survival in seawater for this marine pathogen, we used transposon insertion sequencing analysis to explore the fitness determinants in summer and autumn seawater. Approximately 127 genes linked to metabolism and virulence, as well as other processes, were revealed in E. piscicida to contribute to better adaptations to the seasonal alternations of seawater environments; these genes provide important insights into the infection outbreak mechanisms of E. piscicida and potential improved treatments or vaccines against marine pathogens.