文献类型：期刊文献

英文题名：Study on glucose oxidase fermentation coupled with membrane dialysis

作者：Chu, J; Li, YR; Yu, JT

年份：1997

卷号：67

期号：1-2

起止页码：59

外文期刊名：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:A1997XY76200005)】；

语种：英文

外文关键词：glucose oxidase; fermentation; membrane dialysis; Penicillium sp.; catabolite repression

摘要：The repression of glucose oxidase (GOD) biosynthesis by catabolites during fermentation can be alleviated through membrane dialysis fermentation (MDF). The results show that the volumetric enzyme productivity of MDF was two times higher than that of the control (fermentation without dialysis), and its total enzyme activity was increased by 30-50%. The operation conditions of MDF, such as pore size of the membrane, initiating time for membrane dialysis, and volume of dialysate used, were optimized. The content of amino acids and organic acids in the fermentation broth and the dialysate in the reservoir were assessed by amino acid analyzer and ionic chromatography, respectively. The relationship among the contents of pyruvic acid, gluconic acid, and enzyme activity during fermentation was analyzed quantitatively. Furthermore, the effect of membrane dialysis technology applied to the low-yield strain was found to be more effective than that applied to the high-yield strain.