文献类型：期刊文献

英文题名：Genetic characterizations of Cryptosporidium spp. and Giardia duodenalis in humans in Henan, China

作者：Wang, Rongjun[1];Zhang, Xiaosan[1];Zhu, Huili[1];Zhang, Longxian[1,2,3];Feng, Yaoyu[4];Jian, Fuchun[1];Ning, Changshen[1];Qi, Meng[1];Zhou, Yang[1];Fu, Kanda[5];Wang, Yaqiang[5];Sun, Yanru[1];Wang, Qiang[1];Xiao, Lihua[3]

机构：[1]Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450002, Peoples R China;[2]Atlanta Res & Educ Fdn, Decatur, GA 30033 USA;[3]Ctr Dis Control & Prevent, Div Foodborne Waterborne & Environm Dis, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA 30333 USA;[4]E China Univ Sci & Technol, Sch Resource & Environm Engn, Shanghai 200237, Peoples R China;[5]Henan Univ, Inspect Div Huaihe Hospital, Kaifeng 475000, Peoples R China

年份：2011

卷号：127

期号：1

起止页码：42

外文期刊名：EXPERIMENTAL PARASITOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000286486800008)】；

基金：This study was supported in part by Key National Science & Technology Special Projects (No. 2008ZX10004-011), the State Key Laboratory of Veterinary Etiological Biology, Ministry of Health Special Funds of Public Sector Research (No. 200808012), and the National Natural Science Foundation of China (No. 30928019).

语种：英文

外文关键词：Cryptosporidium; Giardia duodenalis; Humans; Genotyping; Subtyping; Cryptosporidium hominis; Assemblage A; Assemblage B

摘要：Cryptosporidium and Giardia infections are common causes of diarrhea worldwide. To better understand the transmission of human cryptosporidiosis and giardiasis in Henan, China, 10 Oyprosporidium-positive specimens and 18 Giardia-positive specimens were characterized at the species/genotype and subtype levels. Cryptosporidium specimens were analyzed by DNA sequencing of the small subunit rRNA and 60 kDa glycoprotein genes. Among those genotyped, nine belonged to C hominis and one C fells, with the former belonging to three subtype families: Ia, Ib, and Id. The three Ib subtypes identified, IbA16G2, IbA9G2, and IbA20G2, were very different from the two common Ib subtypes (IbA9G3 and IbA10G2) found in other areas of the world. The distribution of Giardia duodenalis genotypes and subtypes was assessed by sequence analysis of the triosephosphate isomerase (tpi) gene. The assemblages A (eight belonging to A-I and four A-II) and B (belonging to six new subtypes) were found in 12 and six specimens, respectively. More systematic studies are needed to understand the transmission of Cryptosporidium and G. duodenalis in humans in China. Published by Elsevier Inc.