文献类型：期刊文献

英文题名：An individually coated near-infrared fluorescent protein as a safe and robust nanoprobe for in vivo imaging

作者：Yang, Yu[1];Xiang, Kun[1];Yang, Yi-Xin[1];Wang, Yan-Wen[1];Zhang, Xin[1];Cui, Yangdong[1];Wang, Haifang[1];Zhu, Qing-Qing[2];Fan, Liqiang[2];Liu, Yuanfang[3];Cao, Aoneng[1]

机构：[1]Shanghai Univ, Inst Nanochem & Nanobiol, Shanghai 200444, Peoples R China;[2]E China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China

年份：2013

卷号：5

期号：21

起止页码：10345

外文期刊名：NANOSCALE

收录：;EI(收录号：20134316877127);WOS:【SCI-EXPANDED(收录号:WOS:000325762000033)】；

基金：This work was supported by the National Basic Research Program of China (973 Program no. 2009CB930200 and 2011CB933402), the China Natural Science Foundation (no. 21371117), Key Project of Chinese Ministry of Education (211053), Shanghai Municipal Education Committee (11ZZ82), and Shanghai Leading Academic Disciplines (S30109).

语种：英文

外文关键词：Quantum yield - Probes - Fluorescence imaging - Proteins - Infrared devices - Silica

摘要：A prerequisite for in vivo fluorescence imaging is the safety of fluorescent probes. Among all fluorescent probes, fluorescent proteins (FPs) might be the safest ones, which have been widely used in biological sciences at the gene level. But FPs have not been used in vivo in the purified form yet due to the instability of proteins. Here, we individually coat near-infrared (NIR) FPs (NIRFPs) with a silica nanoshell, resulting in NIRFP@silica, one of the safest and brightest NIR fluorescent nanoprobes with a quantum yield of 0.33 for in vivo imaging. The silica shell not only protects NIRFPs from denaturation and metabolic digestion, but also enhances the quantum yield and photostability of the coated NIRFPs. When injected via the tail vein, NIRFP@silica NPs can distribute all over the mouse body, and then can be efficiently eliminated through urine in 24 h, demonstrating its potential applications as a safe and robust NIR fluorescence probe for whole body imaging.