文献类型：期刊文献

中文题名：固定化酵母细胞连续生产转化糖反应动力学的研究

英文题名：A Mathematic Model for the Continuous Production Invert Sugar with Immobilized Yeast Cells

作者：李凡超[1];徐京宁[1];张嗣良[1];俞俊棠[1]

机构：[1]华东化工学院生化工程研究所

年份：1992

卷号：8

期号：4

起止页码：378

中文期刊名：生物工程学报

外文期刊名：Chinese Journal of Biotechnology

收录：CSTPCD;;Scopus;北大核心:【北大核心1992】；CSCD:【CSCD2011_2012】；PubMed;

语种：中文

中文关键词：转化糖;固定化细胞;动力学;酵母

外文关键词：Mathematic model; invert sugar; immobilized cells; surgar; kinetics

摘要：利用海藻酸钠固定化面包酵母细胞,在搅拌罐和填充柱反应器内连续反应由蔗糖生产转化糖,对该过程的反应动力学研究结果表明:内、外传质阻力,转化酶活力、料液流量,搅拌速率和媒体半径对转化率有影响,本文给出了它们之间相互关系的数学表达式,本文还定义了阻力因子,动力因子及返混因子,并讨论了它们对转化效率所起的作用,给出了串联反应器操作的有关计算公式,测定了有关动力学参数,给出了料液流量控制,搅拌转速控制,媒体半径选择和最大转化率操作条件的计算方法,并在搅拌罐反应器、填充柱反应器和串联搅拌罐反应器中进行了实验和计算,从实验上验证了动力学理论研究结果。
A mathematic model is proposed for the continuous production invent sugar from sucrose by immobilized yeast cells. The yeast cell are immobilized in Ca^(2+)-alginate and invert sugar is produced in a continuous stirred tank reactor and in a packed bed reactor. The effects of reaction solution flow rate, stirring speed, immobilized cells size and sucrose concentration on the invert rate are given in formula. The reaction kinetic factor, resistance factor and back-mixing factor has been defined and the relationship between invert rate and these factors has been studied. The formulas about multi-stage reactor's operation has been given. The dynemic parameters of the model have been determined. The enzyme activety and the mass diffusion upon the invert rate has been studied and discussed. The principle and method is given which can be used for the control of solution flow rate, the control of stirring speed, the selection of immobilized cell size and the calculation of operation condition for maximum invert rate. The experimental and the calculated results is reported. The mathematic model has been demonstrated by experimental results.