文献类型：专利

英文题名：Producing edible mushroom polysaccharide by enzyme pretreatment-microwave-assisted extraction-chitosan flocculation and purification comprises enzymatic hydrolyzing the edible mushroom powder, microwave irradiating, and flocculating

作者：HAN L;HAN W;LUO W;MA D;MAN N;DOU X;WANG J

机构：[1]UNIV EAST CHINA SCI & TECHNOLOGY

申请号：CN104497159-A

申请日：2014-12-31

公开日：2015-04-08

语种：英文

收录：DERWENT

摘要：NOVELTY - Producing edible mushroom polysaccharide by enzyme pretreatment-microwave-assisted extraction-chitosan flocculation and purification comprises adding the enzyme into the edible mushroom powder, mixing, carrying out enzymatic hydrolysis to obtain a mixture; transferring the mixture into a microwave extraction device, microwave irradiating, cooling, and vacuum filtering to obtain edible mushroom polysaccharide extract; and concentrating the extract, adding chitosan, stirring, flocculating, purifying, centrifuging. USE - The method is useful for producing edible mushroom polysaccharide by enzyme pretreatment-microwave-assisted extraction-chitosan flocculation and purification (claimed). ADVANTAGE - The method saves energy and solvent, removes impurities from high molecular weight protein, has good edulcoration effect, is economical, and does not affect the product quality. DETAILED DESCRIPTION - Producing edible mushroom polysaccharide by enzyme pretreatment-microwave-assisted extraction-chitosan flocculation and purification comprises (i) reflux pre-treating edible mushroom using ethanol, pulverizing the pre-treated edible mushroom to obtain edible mushroom powder, and drying; (ii) adding the enzyme into the edible mushroom powder, mixing, carrying out enzymatic hydrolysis by adding water as solvent, to obtain a mixture; (iii) transferring the mixture into a microwave extraction device, microwave irradiating for 1-4 minutes, cooling at room temperature, and vacuum filtering to obtain edible mushroom polysaccharide extract; and (iv) concentrating the extract, adding chitosan, stirring, flocculating, purifying, centrifuging to obtain purified edible mushroom polysaccharide solution, and drying.