文献类型：期刊文献

英文题名：Primers and copper responsive promoter design and data of real-time RT-PCR assay in filamentous fungus Trichoderma reesei

作者：Wang, Wei[1];Chen, Yumeng[1];Wei, Dong-Zhi[1]

机构：[1]East China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China

年份：2018

卷号：16

起止页码：109

外文期刊名：DATA IN BRIEF

收录：EI(收录号：20224112873537);WOS:【ESCI(收录号:WOS:000449758500019)】；

基金：This research was supported by the National Natural Science Foundation of China (No. C010302-31500066) and the Fundamental Research Funds for the Central Universities (No. 222201714053).

语种：英文

外文关键词：Trichoderma reesei; Filamentous fungus; Copper responsive promoter; Quantitative real-time PCR; Plasmid construction; Gene expression

摘要：This data article contains data related to the research article entitled "Copper-mediated on-off control of gene expression in filamentous fungus Trichoderma reesei" (Wang et al., 2017) [1]. Four kinds of copper responsive promoters were designed. Quantitative PCR (qPCR) was performed to determine relative mRNA levels of red fluorescent protein gene (rfp) extracted from cells grown under different concentrations of CuSO4. Three deletion vectors were constructed by using a copper-mediated self-excision cassette instead of a xylose-mediated self-excision cassette (Zhang et al., 2016) [2] to knock out xynl, one of the two major specific endo-beta-1,4-xylanases (Rauscher et al., 2006) [3], xyrl, the key transcriptional activator of cellulolytic and xylanolytic genes (Lichius et al., 2015) [4], and ace3, a factor essential for cellulase production (Hakkinen et al., 2014) [5]. This data article reports the primers, vector construction, and qPCR assay. (C) 2017 The Authors. Published by Elsevier Inc.