文献类型：期刊文献

英文题名：Preparation and evaluation of 3 m open tubular capillary columns with a zwitterionic polymeric porous layer for liquid chromatography

作者：Peng, Li[1];Zhu, Manman[1];Zhang, Lingyi[1];Liu, Haiyan[1];Zhang, Weibing[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2016

卷号：39

期号：19

起止页码：3736

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20164102898955);WOS:【SCI-EXPANDED(收录号:WOS:000389044300010)】；

基金：We gratefully acknowledge the support of the National Key Scientific Instrument and Equipment Development Project (2012YQ120044), the State Key Program of National Natural Science of China (21235005), and the National Natural Science Foundation of China (no.21475044).

语种：英文

外文关键词：Liquid chromatography; Open tubular columns; Porous layers; Zwitterionic polymers

摘要：A 3 m zwitterionic polymeric porous layer open tubular column (3 m x 25 mu m id x 375 mu m od) with a polymeric porous layer thickness of 4 mu m was fabricated by the copolymerization of [2-(methacryloyloxy) ethyl] dimethyl-(3-sulfopropyl) ammonium hydroxide and N,N'-methylenebis(acrylamide). The effects of the diameter of the capillary, reaction temperature, and polymerization time on the preparation of the open tubular column were investigated. Characterized by scanning electron microscopy, the zwitterionic layer was observed to be rough and throughout the fused-silica capillary homogenously, which increased the phase ratio. The separation of neutral, basic, and acidic compounds demonstrates the strong hydrophilicity of the poly[2-(methacryloyloxy) ethyl] dimethyl-(3-sulfopropyl) ammonium hydroxide coating. In addition, the poly[2-(methacryloyloxy) ethyl] dimethyl-(3-sulfopropyl) ammonium hydroxide porous layer open tubular column was applied for the analysis of flavonoids from the rootstalk of licorice, revealing the potential in separating complex samples. The relative standard deviation of retention time for run-to-run (n = 5), day-to-day (n = 3), and column-to-column (n = 3) of toluene, N, N-dimethylformamide, formamide, and thiourea were below 1.2%, exhibiting good repeatability.