文献类型：期刊文献

英文题名：Characterization and application of D-amino acid oxidase and catalase within permeabilized Pichia pastoris cells in bioconversions

作者：Tan, Qiang; Song, Qingxun; Zhang, Yewang; Wei, Dongzhi

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, New World Inst Biotechnol, Shanghai 200237, Peoples R China

年份：2007

卷号：136

期号：3

起止页码：279

外文期刊名：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000245961200005)】；

语种：英文

外文关键词：D-amino acid oxidase; catalase; stability; permeabilized cells; bioconversion

摘要：The high-density fermentation of recombinant Pichia pastoris was carried out in a 1-L fermentor. After 60 h of fermentation, the activities Of D-amino acid oxidase (DAAO) and catalase assayed with the permeabilized cells attained 12,532 and 684,800 U/L, respectively. Additionally, the stability of DAAO and catalase within the permeabilized. cells was relatively high. The half-life of the two enzymes reached 14.5 and 4.0 d at 30 degrees C, respectively. Furthermore, these permeabilized cells could convert D-phenylalanine into 99% phenylpyruvate within 100 min and could be efficiently reused up to 13 cycles. After being treated with base and heating, these treated permeabilized cells could be reused up to three cycles in a batchwise conversion of cephalosporin C, and about 90% 7-beta-(4-carboxybutanamido)-cephalosporanic acid was ultimately obtained at each cycle.