文献类型：期刊文献

英文题名：From energy sensing to epigenetic activation: The AMPK/ TrSnf1-ACE3-MST2 pathway orchestrates cellulase synthesis in Trichoderma reesei

作者：Chen, Yumeng[1,2];Gao, Xin[1,2];Ding, Jie[1,2];Qiu, Zhouyuan[1,2];Fan, Xingjia[1,2];Zhao, Xihua[3];Wei, Dongzhi[1,2];Wang, Wei[1,2,4]

机构：[1]State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Luhua Biotechnol Res Inst, Shanghai 200237, Peoples R China;[3]Jiangxi Normal Univ, Coll Life Sci, Nanchang, Peoples R China;[4]Tianjin Inst Ind Biotechnol, Tianjin, Peoples R China

年份：2026

卷号：365

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20261920669348);WOS:【SCI-EXPANDED(收录号:WOS:001766397600001)】；

基金：We thank professor Qun He (College of Biological Sciences, China Agricultural University) for providing the ACE3 polyclonal antibody. This work was supported by the National Key Research and Devel-opment Program of China [grant no. 2022YFA0912300] ; National Natural Science Foundation of China [no. 32370093, 22578121, and 32000050] ; Natural Science Foundation of Shanghai [no. 23ZR1414800] ; and the Open Funding Project of the State Key Labo-ratory of Bioreactor Engineering [no. 2022] .

语种：英文

外文关键词：Trichoderma reesei; AMPK/Snf1; Histone acetyltransferases

摘要：Fungal gene expression often relies on energy-sensing kinase Snf1 and histone acetyltransferase, particularly for highly expressed or rapidly modulated genes such as those encoding cellulases. Cellulase production by Trichoderma reesei is of great industrial importance; however, most studies on Snf1 function have focused on yeast. This study aimed to determine the mechanisms underlying the rapid response of the Snf1 ortholog in T. reesei (TrSnf1) to carbon source depletion and the subsequent activation of cellulase gene transcription. TrSnf1 senses glucose depletion relieves carbon catabolite repression (CCR) and ultimately triggers cellulase gene expression. Multi-omics analysis identified the histone acetyltransferase MST2 and highlighted the regulatory role of its interaction with TrSnf1 in cellulase expression. MST2 deletion impairs cellulase gene expression, suggesting that its interaction with MST2 is critical for its regulatory function. MST2 is involved in histone H3 acetylation and RNA polymerase binding to cellulase gene promoters. We propose that the TrSnf1-MST2-ACE3 regulatory pathway orchestrates the transcriptional activation of cellulase. This study addresses a critical gap in the CCR regulatory network of T. reesei, specifically the persistent CCR observed in CRE1-knockout strains. Our study uncovered a novel CRE1-independent CCR signaling pathway and provides a theoretical foundation for developing high-yield cellulase strains through genetic engineering.