文献类型：期刊文献

中文题名：培养基组分和发酵温度对重组酵母表达HBsAg的影响

英文题名：EFFECTS OF CULTURE MEDIA COMPOSITION AND TEMPERATURE ON EXPRESSION OF HBsAg BY RECOMBINANT YEAST IN FERMENTATION PROCESSES

作者：施源[1];唐孝宣[1];袁渭康[1];陈敏恒[1];沈绿萍[2];李载平[2]

机构：[1]华东化工学院;[2]中国科学院上海生物化学研究所;

年份：1989

卷号：5

期号：3

起止页码：207

中文期刊名：生物工程学报

外文期刊名：Chinese Journal of Biotechnology

收录：CSTPCD;;Scopus;北大核心:【北大核心1992】；CSCD:【CSCD2011_2012】；PubMed;

语种：中文

中文关键词：重组酵母;工程菌发酵;乙型肝炎;表面抗原

外文关键词：Fermentation by recombinant yeast; hepatitis B surface antigen; stability of recombinant plasmid

摘要：分析酵母基因工程菌生产目的基因表达产物——乙型肝炎表面抗原的发酵过程,降低培养基中葡萄糖浓度,补充甘油和蔗糖,能使乙型肝炎表面抗原相对浓度从3.89提高至8.12,比活值增加2.69倍。根据实验观察的结果,造成发酵过程的温度序列,能合理地分配受体细胞代谢能量的消耗,使目的基因的表达量提高至13.51相对浓度。同时,对流加式操作系统中表现出的重组质粒稳定性的变化规律给予描述和解释。另外,提出了异源DNA与受体细胞的转化过程存在着一个分布,此分布对通过发酵提高乙型肝炎表面抗原的产量造成影响。
Fermentation was performed to produce exogenous protein-hetatitis B surface antigen (HBsAg) by recombinant ycast S. cerevisiae containing the HBsAg gene. These studies showed that relative concentrations of HBsAg were increased from 3.89 to 8.12 when glucese content was reduced and glycerol and sucrose were complemented in culture media, and also, specific activity was increased 2.09-fold. According to the results of experiments, the sequence of temperature variation in fermentation processes was set up. Then the expressed amount of HBsAg gene achieved 13.51 in relative concentration. The stability of a recombinant plasmid containing HBsAg gene was observed and explained by altering fermentation temperatures in fed-batch operation. On the other hand, there is a distribution within transformation between exogcnous DNA and host yeast and it will affect the production of HBsAg by recombinant yeast in fermentation processes.