文献类型：期刊文献

英文题名：Multiple effects of sodium dodecyl sulfate on chromogenic catalysis of tetramethylbenzidine with horseradish peroxidase

作者：Li, Meng[1,2];He, Yifan[1];Meng, Hong[1];Dong, Yinmao[1];Shang, Yazhuo[2];Liu, Honglai[2];Qu, Zhaohui[3];Liu, Youting[3]

机构：[1]Beijing Technol & Business Univ, Sch Sci, Key Lab Cosmet, China Natl Light Ind, Beijing 100048, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[3]Nutriwoods Biotech Beijing Co Ltd, Beijing, Peoples R China

年份：2021

卷号：42

期号：4

起止页码：526

外文期刊名：JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY

收录：;EI(收录号：20195207929966);WOS:【SCI-EXPANDED(收录号:WOS:000503552700001)】；

基金：This work is supported by the Open Research Fund Program of Key Laboratory of Cosmetic (Beijing Technology and Business University), China National Light Industry (Project No. KLC-2018-ZD1) and the National Natural Science Foundation of China (Project No. 21476072).

语种：英文

外文关键词：Horseradish peroxidase (HRP); tetramethylbenzidine (TMB); sodium dodecyl sulfate (SDS); kinetic mechanism; chromogenic catalysis

摘要：In this work, the chromogenic catalysis of 3,3',5,5'-tetramethylbenzidine (TMB) with horseradish peroxidase (HRP) in presence of hydrogen peroxide (H2O2), the most widely used chromogenic reaction in enzyme linked immunosorbent assay (ELISA) was deeply studied in the medium containing sodium dodecyl sulfate (SDS). The effects of SDS on the catalytic efficiency and the kinetic behaviors of chromogenic catalysis of HRP-H2O2-TMB were systematically investigated. It was found that the catalysis of HRP-H2O2-TMB follows a ping-pong kinetic mechanism in SDS solutions based on the theoretical model of double substrates enzymatic kinetics. Particularly, multiple significant effects of SDS in different reaction stages have been summarized as follows: the solubilizer of TMB at the early stage of reaction, the promoter of electron transfer at the happening stage of reaction, the stabilizer of blue chromogens at the end of the reaction as well as the binder of HRP during the course of reaction. The results have shown that the first three effects of SDS prevail when SDS concentration is less than 2.5 mM and leading to the catalytic efficiency increase significantly with SDS concentration. However, once SDS concentration is over 2.5 mM, the bonding of SDS on HRP by the strong electrostatic and hydrophobic interaction render SDS act as inhibitors for HRP. The present work may provide feasible means for purposive tailor the chromogenic catalysis of TMB with HRP and improve its chromogenic performance in biochemical detection.