文献类型：期刊文献

英文题名：Nuclease Activity and Cytotoxicity Enhancement of the DNA Intercalators via Graphene Oxide

作者：Zheng, Bin[1];Wang, Chong[1];Wu, Congyu[2];Zhou, Xuejiao[2];Lin, Min[1];Wu, Xiaochen[2];Xin, Xiaozhen[1];Chen, Xin[1];Xu, Lin[1];Liu, Hui[1];Zheng, Jing[1];Zhang, Jingyan[1];Guo, Shouwu[2]

机构：[1]E China Univ Sci & Technol, Shanghai Key Lab New Drug Design, State Key Lab Bioreactor Engn, Sch Pharm, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Natl Key Lab Micro Nano Fabricat Technol, Key Lab Thin Film & Microfabricat, Minist Educ,Res Inst Micro Nano Sci & Technol, Shanghai 200240, Peoples R China

年份：2012

卷号：116

期号：29

起止页码：15839

外文期刊名：JOURNAL OF PHYSICAL CHEMISTRY C

收录：;EI(收录号：20123115295387);WOS:【SCI-EXPANDED(收录号:WOS:000306725200079)】；

基金：This work was supported by the National Science foundation of China (Nos. 31070742, 91123011, 90923041), the state key laboratory of bioreactor engineering (No. 2060204), 111 Project (No. B07023), the Shanghai Committee of Science and Technology (No. 11DZ2260600), and the National "973 Program" (No. 2010CB933900).

语种：英文

外文关键词：Graphene - DNA

摘要：The enhancement of DNA affinity of small molecules usually ensures their high nuclease activities, and may also open a new scope of their applications in biology and medicine. In this work, we demonstrate that the nuclease activity and cytotoxicity of the small DNA intercalators can be dramatically enhanced by single atomic-layered graphene oxide (GO) sheets. Through pi-pi stacking interaction mainly between GO and the aromatic ligands of intercalators, the conjugates of GO and a small intercalator could be formed. Because of the large planar structure of the GO sheets, the coupling of GO with the small intercalators increased their affinity to DNA. Owing to the formation of conjugates with GO, the binding site of small intercalators to DNA was also changed from a minor groove to a major groove. Notably, GO and small intercalator conjugates exhibited higher cytotoxicity than that of the small intercalator alone. The results open up potential applications of GO for new chemotherapeutic agents that work through DNA intercalation.