文献类型：期刊文献

英文题名：An oral vaccine based on chitosan/aluminum adjuvant induces both local and systemic immune responses in turbot (Scophthalmus maximus)

作者：Jin, Peng[1];Sun, Fei[1];Liu, Qin[1,3];Wang, Qiyao[1,3];Zhang, Yuanxing[2,3];Liu, Xiaohong[1,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519000, Peoples R China;[3]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China

年份：2021

卷号：39

期号：51

起止页码：7477

外文期刊名：VACCINE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000731300100018)】；

基金：This work was financially supported by grants from National Key Research and Development Program of China (2018YFC0311303 to XL) , the National Natural Science Foundation of China (32025038 to QL) , and the Fundamented Research Funds for Central Universities (222201211729) .

语种：英文

外文关键词：Alum adjuvant; Chitosan nanoparticles; Oral vaccine; Vibrio anguillarum

摘要：Oral vaccination is the most convenient method for disease protection in aquaculture due to possibility of adding vaccines as part of fish diet. However, low protective efficiency is the major problem of oral vaccination owing to some reasons, especially for antigens degradation. In this study, we developed an oral inactivated vaccine based on a chitosan/aluminum adjuvant system, and investigated immune response induced by the vaccine. As a result, Th1 and Th2 cells mediated immune responses were observed after the vaccination according to the upregulation of IL-6, IL-12, IFNc and IgM genes expression that were deemed as the driver cytokines for triggering the responses. Moreover, ratio of IgT'/IgM' B cells was elevated in intestine after vaccination, while IgM antibodies were also observed in the sera of vaccinated fish, suggested that both local and systemic immune responses were induced by the inactivated vaccine. Totally, this oral vaccine can be used for V. anguillarum protection and this chitosan/aluminum system is an efficient candidate adjuvant for developing more vaccines in the future. (c) 2021 Elsevier Ltd. All rights reserved.