文献类型：期刊文献

中文题名：高效液相色谱法和毛细管电泳法测定甘草制品中甘草酸的含量

英文题名：Determination of Glycyrrhizic Acid in Glycyrrhiza Preparations with Capillary Electrophoresis and High Performance Liquid Chromatography

作者：彭军[1];王复[1];朱明华[1]

机构：[1]华东理工大学分析测试中心

年份：1999

卷号：17

期号：1

起止页码：90

中文期刊名：色谱

外文期刊名：Chinese Journal of Chromatography

收录：CSTPCD;;Scopus;北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；PubMed;

基金：上海市教委科技基金

语种：中文

中文关键词：测定;MECC;HPLC;CZE;甘草酸;甘草制品

外文关键词：high performance liquid chromatography, capillary zone electrophoresis, micellar electrokinetic capillary chromatography, glycyrrhizic acid, glycyrrhiza preparation

摘要：分别用高效液相色谱法（ＨＰＬＣ）、毛细管区带电泳法（ＣＺＥ）、胶束电动毛细管色谱法（ＭＥＣＣ）测定了甘草制品中甘草酸的含量。对ＨＰＬＣ，ＣＺＥ，ＭＥＣＣ的分析条件作了一些选择实验，结果表明ＭＥＣＣ法与ＨＰＬＣ法分析数据接近、比较准确，而且前者比ＨＰＬＣ法分离效率高、溶剂用量少，是一种很有发展潜力的分析方法。
High performance liquid chromatography (HPLC), capillary zone electrophoresis (CZE) and micellar electrokinetic capillary chromatography(MECC) have been used to determine glycyrrhizic acid in glycyrrhiza preparations. By HPLC, mobile phase was V( methanol )∶V( water ):V( acetic acid )=75∶ 23.5 ∶1.5  By CZE, experiment was performed with 15 kV power, 0.075 mm i.d.×800 mm fused silica capillary column and UV detector. Samples were injected into the capillary by electromigration injection for 20 s. Absorbance detection was at 254 nm. The running buffer was made up of 0.02 mol/L dipotassium hydrogenphosphate and borax (pH 9.0). By MECC, the running buffer was made up of 0.025 mol/L sodium dodecyl sulfate (SDS), 0.02 mol/L dipotassium hydrogenphosphate and borax (pH 9.0). Each new capillary was washed with 0.1 mol/L NaOH, deionized water and buffer, each for 3 min, before use. Comparison of the results of analysis with HPLC, CZE and MECC has been made. It was found that result of MECC was very close to that of HPLC. MECC has been satisfactorily applied to plant drug analysis.