文献类型：期刊文献

英文题名：Characterization of P450 FcpC, the enzyme responsible for bioconversion of diosgenone to isonuatigenone in Streptomyces virginiae IBL-14

作者：Wang, Wei[1]; Wang, Feng-Qing[1]; Wei, Dong-Zhi[1]

机构：[1] State Key Lab. of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China

年份：2009

卷号：75

期号：12

起止页码：4202

外文期刊名：Applied and Environmental Microbiology

收录：EI(收录号：20092512143258)

语种：英文

外文关键词：Escherichia coli

摘要：A new cytochrome P450 monooxygenase, FcpC, from Streptomyces virginiae IBL-14 has been identified. This enzyme is found to be responsible for the bioconversion of a pyrano-spiro steroid (diosgenone) to a rare nuatigenin-type spiro steroid (isonuatigenone), which is a novel C-25-hydroxylated diosgenone derivative. A whole-cell P450 system was developed for the production of isonuatigenone via the expression of the complete three-component electron transfer chain in an Escherichia coli strain. Copyright ? 2009, American Society for Microbiology. All Rights Reserved.