文献类型：期刊文献

英文题名：Synthesis and characterization of anaerobic degradation biomarkers of n-alkanes via hydroxylation/carboxylation pathways

作者：Zhou, Jing[1,2];Bian, Xin-Yu[1,2];Zhou, Lei[1,2];Mbadinga, Serge Maurice[1,2,4];Yang, Shi-Zhong[1,2];Liu, Jin-Feng[1,2];Gu, Ji-Dong[3];Mu, Bo-Zhong[1,2,4]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Inst Appl Chem, Shanghai 200237, Peoples R China;[3]Univ Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China;[4]Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai 200237, Peoples R China

年份：2016

卷号：22

期号：1

起止页码：31

外文期刊名：EUROPEAN JOURNAL OF MASS SPECTROMETRY

收录：;EI(收录号：20194907777472);WOS:【SCI-EXPANDED(收录号:WOS:000370030700004)】；

基金：This work was supported by the National Natural Science Foundation of China (nos. 41530318, 41373070, 41403066) and NSFC-RGC (41161160560).

语种：英文

外文关键词：hydroxylation/carboxylation; potential biomarkers; 2-acetylalkanoic acid; synthesis of biomarkers; mass spectral characteristics

摘要：Metabolite profiling is a powerful method in research on anaerobic biodegradation of hydrocarbons. Hydroxylation and carboxylation are proposed pathways in anaerobic degradation but very little direct evidence is available about metabolites and signature biomarkers. 2-Acetylalkanoic acid is a potential signature metabolite because of its unique and specific structure among possible intermediates. A procedure for the synthesis of four homologues with various carbon chain lengths was proposed and the characteristics of 2-acetylalkanoic acid esters were investigated using four derivatization processes, namely methyl, ethyl, n-butyl and trimethylsilyl esterification. Four intermediate fragments observed were at m/z 73 + 14n, 87 + 14n, 102 + 14n (n = 1, 2 and 4 for methyl, ethyl and n-butyl ester, respectively) and [M - 42](+) for three of the derivatization methods. For silylation, characteristic ions were observed at m/z 73, 117, [M - 42](+) and [M - 55](+). These are basic and significant data for the future identification of potential intermediates of the hydroxylation and carboxylation pathways in hydrocarbon degradation.