文献类型：期刊文献

英文题名：Acclimation process of cultivating Chlorella vulgaris in toxic excess sludge extract and its response mechanism

作者：Wang, Lu[1]; Wang, Hualin[1]; Chen, Xiurong[1]; Zhuang, Youjun[1]; Yu, Zeya[1]; Zhou, TianJun[1]

机构：[1]East China Univ Sci & Technol, State Environm Protect Key Lab Environm Risk Asse, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, NELHROWTT, Natl Engn Lab High Concentrat Refractory Organ Wa, Shanghai 200237, Peoples R China

年份：2018

卷号：628-629

起止页码：858

外文期刊名：SCIENCE OF THE TOTAL ENVIRONMENT

收录：;EI(收录号：20180804825219);WOS:【SCI-EXPANDED(收录号:WOS:000432462000089)】；

基金：This work was supported by the Shanghai Pujiang Program in China [Grant No. 13PJD009]; the National Natural Science Foundation of China [Grant No. 51378207] and the National Science and Technology Major Project of the Ministry of Science and Technology of China (Grant No. 2014ZX07202-011-002). The authors also would like to express their great appreciation to China Scholarship Council (CSC).

语种：英文

外文关键词：Chiorella vulgaris; Toxic excess sludge; Acclimation; Toxicity removal; Response mechanism

摘要：Chiorella vulgaris was cultivated in the gradually increased proportion of toxic sludge extracts for acclimation, which was obtained from SBR treated synthetic wastewater containing mixed chlorophenols (2,4,6-trichlorophenol and 4-chlorophenol). The growth of C. vulgaris was obviously improved after acclimation with the cell number in the 100% sludge group was 22.75 +/- 0.85 * 10(6) cell mL(-1), which was relatively more than the BG11 control group's (20.80 +/- 0.35 * 10(6) cell mL(-1)) and apparently over the 100% sludge group (10.78 +/- 0.45 * 10(6) cell mL(-1)). Compared with the sludge control sludge group, C vulgaris in the aaclimation group gained 24.1% and 18.2% more relative removal rate about TOC and ecotoxicity, respectively. Proteomics analysis showed that protein spots were more clear and centralized and the clarifications of the different protein spots narrowed from 8 to 5 after acclimation. Proteins related to oxidoreducase activity and energy metabolism were over expressed and C. vulgaris could select the metabolic pathways, especially enhanced pyruvate fermentation, TCA cycle, and glycolysis after acclimation, by over accumulating the corresponding vital enzymes. Conclusively, acclimation was a good method to improve the removal ability and growth of C. vulgaris and algae could acclimatize itself to grow upon the toxic sludge extracts by metabolic selection. We suppose acclimation process was a potential method for algae wastewater treatment and algae cultivation without or reduce dilution. (C) 2018 Published by Elsevier B.V.