文献类型：专利

英文题名：Purifying Grifola frondosa polysaccharide comprises crushing Grifola frondosa, extracting, soaking in e.g. deionized water, loading the polysaccharide extract into activated resin column, collecting the eluate and removing protein

作者：HAN L;HAN W;LUO W;MA D;MAN N;DOU X;WANG J

机构：[1]UNIV EAST CHINA SCI & TECHNOLOGY

申请号：CN104497161-A

申请日：2015-01-04

公开日：2015-04-08

语种：英文

收录：DERWENT

摘要：NOVELTY - Purifying Grifola frondosa polysaccharide comprises: (i) crushing the Grifola frondosa, extracting the crushed particles via microwave using deionized water to obtain polysaccharide extract; (ii) soaking the resin in deionized water, sodium hydroxide solution, hydrochloric acid solution, and deionized water respectively; (iii) packing the column with treated resin to form a resin bed, allowing it to stand overnight, passing deionized water via column; and (iv) loading the polysaccharide extract into the activated resin column, collecting the eluate and removing protein. USE - The method is useful for purifying Grifola frondosa polysaccharide (claimed). ADVANTAGE - The method is simple, short, economical and environmentally-friendly; has high efficiency with less energy consumption; and suitable for large scale industrial production. DETAILED DESCRIPTION - Purifying Grifola frondosa polysaccharide comprises: (i) crushing the Grifola frondosa, taking 20-meshes crushed particles and extracting via microwave using deionized water at pH 6 to obtain polysaccharide extract; (ii) repeatedly soaking the resin in deionized water, then soaking with sodium hydroxide solution for 18-24 hours, soaking with hydrochloric acid solution for 18-24 hours, and rinsing with deionized water; (iii) packing the column with treated resin to form a resin bed, allowing it to stand overnight, passing deionized water through the column for inactivation before use; and (iv) loading the polysaccharide extract into the activated resin column, collecting the eluate and removing protein.