文献类型：期刊文献

英文题名：Enhancing erythromycin production by Saccharopolyspora erythraea E3:: sucBA in chemically defined medium

作者：Ke, Xiang[1];Wang, Shuohan[1];Jiang, Xing[1];Li, Xu[1];Chu, Ju[1,2,3];Tian, Xiwei[1,2,3]

机构：[1]East China Univ Sci & Technol, Qingdao Innovat Inst, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Natl Ctr Bioengn & Technol Shanghai, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai 200237, Peoples R China

年份：2026

卷号：442

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20260820124574);WOS:【SCI-EXPANDED(收录号:WOS:001633574500003)】；

基金：This work was financially supported by the National Key Research and Development Program of China (2024YFA0917700) , the National Natural Science Foundation of China (No. 32501316) , the Taishan Scholars Program of Shandong Province (NO.tsqn202312316) , the Shanghai Pilot Program for Basic Research (22TQ1400100-14) , the Shanghai Science and Technology Innovation Action Plan (24HC2810100) , the Natural Science Foundation of Shanghai (23ZR1416500) , the Fundamental Research Funds for the Central Universities (JKV01251708) . Thanks for the financial support from the Arawana Charity Foundation.

语种：英文

外文关键词：Erythromycin; chemically defined medium; targeted metabolomes; fermentation optimization

摘要：Erythromycin, a clinically vital macrolide antibiotic biosynthesized by Saccharopolyspora erythraea, is predominantly produced using complex media derived from agricultural by-products. However, these complex media bring about batch-to-batch variation, complicate downstream purification, and increase the biological oxygen demand (BOD) of wastewater, thereby raising treatment costs and environmental footprints. To address limitations of complex media, this study focused on optimizing erythromycin fermentation by S. erythraea E3::sucBA in a chemically defined medium (CDM). The engineered strain E3::sucBA exhibits enhanced erythromycin synthesis capacity but suffers from a distinct early-stage growth retardation. Through targeted metabolomes analysis, insufficient intracellular glutamate was pinpointed as the key driver of this growth limitation and a tailored approach medium optimization approach was applied. Supplementing 0.5 g/L glutamate to CDM alleviated the lag, accelerating nutrient consumption and raising erythromycin from 930.1 mg/L to 1307.9 mg/L. Furthermore, a dynamic fed-batch concentration scaling fermentation strategy was developed in 5 L bioreactors, and an erythromycin titer of 4065.8 mg/L was achieved with higher cell density. Vitamin and cofactor addition further optimized productivity, resulting in a record 4847.4 mg/L erythromycin in CDM. In conclusion, this study provided an optimization framework for erythromycin fermentation in the CDM, and offered references for