文献类型：期刊文献

英文题名：VqsA controls exotoxin production by directly binding to the promoter of asp in the pathogen Vibrio alginolyticus

作者：Zhang, Jun[1];Hao, Yuan[1];Yin, Kaiyu[1];Mao, Qiaoqiao[1];Xu, Rongjing[3];Zhang, Yuanxing[1,2];Ma, Yue[1,2];Wang, Qiyao[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Engn Res Ctr Maricultured Anim Vaccines, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Yantai Dev Zone TianYuan Aquat Prod Co Ltd, Yantai 264006, Shandong, Peoples R China

年份：2019

卷号：366

期号：6

外文期刊名：FEMS MICROBIOLOGY LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000469764000002)】；

基金：This work was supported by grants from National Natural Science Foundation of China (Nos. 31772893 to YM and 31772891 to QW), the Ministry of Agriculture of China (CARS-47), the Science and Technology Commission of Shandong and Shanghai Municipality (2017CXGC0103 and 17391902000).

语种：英文

外文关键词：Asp; Vibrio alginolyticus; VqsA; QS; virulence

摘要：Quorum sensing (QS) system is an important bacterial cell-to-cell signaling system controlling expression of various genes in response to cell densities. In vibrios, LuxR/AphA are two established master QS regulators (MQSRs), and VqsA is recently identified to be the third putative MQSR. As a novel LysR-type regulator, the regulon and the underlying regulation mechanisms of VqsA remains to be elucidated. Here our investigation indicated that the yields of alkaline serine protease (Asp), the exotoxin in Vibrio alginolyticus was dependent on both LuxR and VqsA in growth phase dependent manner. Various in vivo and in vitro analyses including electrophoretic mobility shift assays (EMSA) along with DNase I footprinting investigations demonstrated that VqsA positively controls asp expression through directly binding to the partially palindromic 29 bp binding motif in the promoter region of asp. Moreover, RNA-seq analysis validated the regulatory roles of VqsA in various processes in the organism. Collectively, our data showed that VqsA positively regulates the expression of exotoxin and other virulence-associated genes and is essential for the QS regulation in V. alginolyticus.