文献类型：期刊文献

中文题名：高产耐热3′-磷酸二酯酶菌种的选育及酶性质的研究

英文题名：Screening of Strains Overproducing Thermostable 3′-Phosphodiesterase and Its Property

作者：修俊鹏[1];丁庆豹[1];欧伶[1];邱蔚然[2]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237;[2]上海秋之友生物科技有限公司,上海201612

年份：2006

卷号：32

期号：3

起止页码：274

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;EI(收录号：2006189857111);Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：3′-磷酸二酯酶;3′-核苷酸;粘红酵母;诱变育种

外文关键词：3′-phosphodiesterase ; 3′-ribonucleotides ; Rhodotorula glutinis ; UV-mutation

摘要：以粘红酵母(Rhodotoru la g lutin is)Q 0402为出发菌株,经紫外诱变,得到一株粘红酵母QX 0402。5 L发酵罐的发酵液中3′-磷酸二酯酶酶活力可达250 U/mL,为文献报道过的最高酶活力的2.5倍。所产生的3′-磷酸二酯酶在70°C下保温1 h,酶活力仍有50%以上。该酶最适反应pH为5.4,最适反应温度为60°C,反应3 h的反应转化率为56.37%,底物浓度可以提高到3%,生产效率为文献报道的3倍。
A Rhodotorula glutinis QX0204 which could produce 250 U/mL thermostable 3′-phosphodiesterase in a 5L fermenter was obtained from Q0204 through ultraviolet irradiation. Being incubated at 70 ℃ for 1 h 3′-phosphodiesterase activity still attained over 50%. Its optimal pH and temperature was 5.4 and 60℃ respectively. The yield of 3′-ribonucleotides reached 56. 37% at 60℃ after 3 h reaction. Substrates concentration used could be increased to 3′ and the productivity was 3 times more than that reported.