文献类型：期刊文献

英文题名：Asymmetric bioreduction of γ- and δ-keto acids by native carbonyl reductases from Saccharomyces cerevisiae

作者：Ren, Chunlei[1];Wang, Tao[1];Zhang, Xiaoyan[1];Pan, Jiang[1];Xu, Jianhe[1,2];Bai, Yunpeng[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg, Shanghai 200237, Peoples R China

年份：2021

卷号：29

起止页码：305

外文期刊名：CHINESE JOURNAL OF CHEMICAL ENGINEERING

收录：;EI(收录号：20205209695731);WOS:【SCI-EXPANDED(收录号:WOS:000636564100036)】；

基金：This work was financially sponsored by the National Key Research and Development Program of China (2016YFA0204300, 2019YFA09005000), the National Natural Science Foundation of China (21536004, 21776085, 21871085), the Natural Science Foundation of Shanghai (18ZR1409900), Key Project of the Shanghai Science and Technology Committee (18DZ1112703) and the Fundamental Research Funds for the Central Universities (WF1714026).

语种：英文

外文关键词：Keto acids/esters; (R)-gamma-/delta-Decalactones; Carbonyl reductase; Asymmetric reduction; Saccharomyces cerevisiae

摘要：Optically pure (R)-gamma- and (R)-delta-lactones can be prepared by intramolecular cyclization of chiral hydroxy acids/esters reduced asymmetrically from gamma- and delta-keto acids/esters using Saccharomyces cerevisiae (S. cerevisiae) as a whole-cell biocatalyst. However, some of the enzymes catalyzing these reactions in S. cerevisiae are still unknown up to date. In this report, two carbonyl reductases, OdCR1 and OdCR2, were successfully discovered, and cloned from S. cerevisiae using a genome-mining approach, and overexpressed in Escherichia coli (E. coli). Compared with OdCR1, OdCR2 can reduce 4-oxodecanoic acid and 5-oxodecanoic acid asymmetrically with higher stereoselectivity, generating (R)-gamma-decalactone (99% ee) and (R)-delta-decalactone (98% ee) in 85% and 92% yields, respectively. This is the first report of native enzymes from S. cerevisiae for the enzymatic synthesis of chiral gamma- and delta-lactones which is of wide uses in food and cosmetic industries. (C) 2020 The Authors. The Chemical Industry and Engineering Society of China, and Chemical Industry Press Co., Ltd. All rights reserved.