文献类型：期刊文献

中文题名：桑叶总黄酮的大孔树脂纯化工艺

英文题名：Purification of total flavones from Mulberry Leaves by macroporous resin

作者：花蕾[1];张文清[1];夏玮[1]

机构：[1]华东理工大学化学与分子工程学院,上海200237

年份：2007

卷号：29

期号：12

起止页码：1758

中文期刊名：中成药

外文期刊名：Chinese Traditional Patent Medicine

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：大孔树脂;桑叶总黄酮;工艺优化

外文关键词：macroporous resin ; total flavonoes of Mulberry Leaves ; optimization of technics

摘要：目的:选择8种大孔吸附树脂分别对桑叶总黄酮进行静态与动态的吸附与解吸,筛选纯化桑叶总黄酮的最佳树脂。方法:研究影响分离的各种因素如流速、上柱浓度、pH和洗脱剂等优化分离工艺。结果:研究结果表明:AB-8树脂宜于桑叶总黄酮的提纯,最佳工艺为:流速为2BV/h,上样桑叶总黄酮水溶液浓度为0.613mg/mL,pH值为4,乙醇为洗脱剂,洗脱浓度为70,树脂每使用3次进行一次再生。结论:经AB-8树脂吸附分离后,黄酮含量提高1倍以上。
AIM： To sieve the optimal purification of the total flavonoid from Mulberry Leaves by exploring 8 types of macroporous resin＇ s static and dynamic adsoption-desorption. METHODS： The factors affecting separation, such as flow rate concentration, pH and kind of eluting agent were considerated. RESULTS ： Experiment results showed that AB-8 resin possesses high absorption capacity. The best craft was： the adsorption and desorptionpower was 2 BV/h, the content of the total flavonoid in liquid was 0. 613 mg per 1 mL, pH 4, as the desorption solvent, the concentration of ethanol was 70%, regenerate the resin after using three times. CONCLUSION： Flavones content used the method can be up 2-fold.