文献类型：期刊文献

英文题名：Biosynthesis and uptake of glycine betaine as cold-stress response to low temperature in fish pathogen Vibrio anguillarum

作者：Ma, Yue[1,2];Wang, Qiyao[1,2,3];Gao, Xiating[1];Zhang, Yuanxing[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg, Shanghai 200237, Peoples R China

年份：2017

卷号：55

期号：1

起止页码：44

外文期刊名：JOURNAL OF MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000390979000006)】；

基金：This work was supported by grants from the National Natural Science Foundation of China (31272701), Project of Marine Public Welfare Profession (201405003-2), and the Ministry of Agriculture of China (No. CARS-50 and nyhyzx-201303047). We thank especially Cheng Wang for providing the genome data of Vibrio anguillarum MVM425. We thank Miao Guo and Yalin Li at the Nuclear Magnetic Resonance Core Facility, The Institute of Fine Chemical, East China University of Science and Technology, for help and assistance with NMR analysis.

语种：英文

外文关键词：compatible solute; glycine betaine; protectant; cold-stress; Vibrio anguillarum

摘要：Fish pathogen Vibrio anguillarum, a mesophile bacterium, is usually found in estuarine and marine coastal ecosystems worldwide that pose a constant stress to local organism by its fluctuation in salinity as well as notable temperature change. Though V. anguillarum is able to proliferate while maintain its pathogenicity under low temperature (5-18 degrees C), so far, cold-adaption molecular mechanism of the bacteria is unknown. In this study, V. anguillarum was found possessing a putative glycine betaine synthesis system, which is encoded by betABI and synthesizes glycine betaine from its precursor choline. Furthermore, significant up-regulation of the bet gene at the transcriptional level was noted in log phase in response to cold-stress. Moreover, the accumulation of betaine glycine was only found appearing at low growth temperatures, suggesting that response regulation of both synthesis system and transporter system are cold-dependent. Furthermore, in-frame deletion mutation in the two putative ABC transporters and three putative BCCT family transporters associated with glycine betaine uptake could not block cellular accumulation of betaine glycine in V. anguillarum under cold stress, suggesting the redundant feature in V. anguillarum be table transporter system. These findings confirmed that glycine betaine serves as an effective cold stress protectant and highlighted an underappreciated facet of the acclimatization of V. anguillarum to cold environments.