文献类型：期刊文献

英文题名：Selective FRET nano probe based on carbon dots and naphthalimide-isatin for the ratiometric detection of peroxynitrite in drug-induced liver injury

作者：Wu, Yueci[1]; Sun, Lu-Lu[3,4]; Han, Hai-Hao[3,4]; He, Xiao-Peng[5,6]; Cao, Weiguo[2]; James, Tony D.[1,7]

机构：[1] Department of Chemistry, University of Bath, Bath, BA2 7AY, United Kingdom; [2] Department of Chemistry, Shanghai University, Shanghai, 200444, China; [3] Shandong Laboratory of Yantai Drug Discovery, Bohai Rim Advanced Research Institute for Drug Discovery, Shandong, Yantai, 264117, China; [4] Molecular Imaging Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China; [5] Key Laboratory for Advanced Materials, Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, Frontiers Center for Materiobiology and Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Rd, Shanghai, 200237, China; [6] The International Cooperation Laboratory on Signal Transduction, National Center for Liver Cancer, Eastern Hepatobiliary Surgery Hospital, Shanghai, 200438, China; [7] School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, 453007, China

年份：2023

卷号：15

期号：2

起止页码：757

外文期刊名：Chemical Science

收录：EI(收录号：20240115309089)

语种：英文

外文关键词：Carbon - Diseases - Nanoprobes - Probes

摘要：Drug-induced liver injury (DILI) is the most common cause for acute liver failure in the USA and Europe. However, most of DILI cases can recover or be prevented if treatment by the offending drug is discontinued. Recent research indicates that peroxynitrite (ONOO?) can be a potential indicator to diagnose DILI at an early stage. Therefore, the establishment of an assay to detect and track ONOO? in DILI cases is urgently needed. Here, a FRET-based ratiometric nano fluorescent probe CD-N-I was developed to detect ONOO? with high selectivity and excellent sensitivity. This probe consists of carbon dots and a naphthalimide-isatin peroxynitrite sensing system assembled based on electrostatic interactions. Using CD-N-I we were able to detect exogenous ONOO? in live cells and endogenous ONOO? in APAP-induced liver injury of HepG2 cells. ? 2024 The Royal Society of Chemistry.