文献类型：期刊文献

英文题名：Microfluidic-Based dsRNA Delivery Nanoplatform for Efficient Spodoptera exigua Control

作者：Xie, Jinshan[1];Zhang, Jiaxin[1];Yang, Jingyi[1];Wu, Shuqin[1];Teng, Xuanming[1];Han, Hongyu[1];Xu, Yufang[1];Qian, Xuhong[1,2];Zhu, Weiping[1,3,4];Yang, Yangyang[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Sch Pharm, Minist Educ,Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Sch Pharm, Shanghai 200237, Peoples R China

年份：2024

卷号：72

期号：22

起止页码：12508

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20242216175564);WOS:【SCI-EXPANDED(收录号:WOS:001231816100001)】；

基金：This work was supported by the National Key Research and Development Program (2022YFD1700403 and 2023YFD1700303). Prof. Zhiping Xu is acknowledged for providing experimental suggestions and engaging in insightful discussions.

语种：英文

外文关键词：nanopesticide; RNA interference; microfluidictechnology; lipid nanoparticle; Spodopteraexigua

摘要：Nanotechnology-based RNA interference (RNAi) offers a promising approach to pest control. However, current methods for producing RNAi nanopesticides are mainly implemented in a batch-to-batch manner, lacking consistent quality control. Herein, we present a microfluidic-based nanoplatform for RNA nanopesticide preparation using lipid nanoparticles (LNPs) as nanocarriers, taking advantage of the enhanced mass transfer and continuous processing capabilities of microfluidic technology. The dsRNA@LNPs were rapidly formed within seconds, which showed uniform size distribution, improved leaf wettability, and excellent dispersion properties. The delivery efficiency of dsRNA@LNPs was evaluated by targeting the chitin synthetase B (CHSB) gene ofSpodoptera exigua. The dsRNA@LNPs can effectively resist nuclease-rich midgut fluid degradation. Importantly, dsCHSB@LNPs exhibited increased mortality rates, significant reduction of larvae growth, and enhanced gene suppression efficiency. Therefore, a continuous nanoplatform for RNAi nanopesticide preparation is demonstrated by utilizing microfluidic technology, representing a new route to produce RNAi nanopesticides with enhanced quality control and might accelerate their practical applications.