文献类型：期刊文献

中文题名：A suture-free, shape self-adaptive and bioactive PEG-Lysozyme implant for Corneal stroma defect repair and rapid vision restoration

作者：Hang Zhou[1];Shaohua Zhang[2];Miao Lei[1];Yixin Cai[1];Honglei Wang[1];Jianguo Sun[2];Jingyuan Cui[1,3];Changsheng Liu[1];Xue Qu[1,3,4]

机构：[1]Key Laboratory for Ultrafine Materials of Ministry of Education,School of Material Science and Engineering,Frontiers Science Center for Materiobiology and Dynamic Chemistry,East China University of Science and Technology,Shanghai,200237,China;[2]Eye Institute and Department of Ophthalmology,NHC Key Laboratory of Myopia(Fudan University),Key Laboratory of Myopia,Chinese Academy of Medical Sciences,Shanghai Key Laboratory of Visual Impairment and Restoration,Eye&ENT Hospital,Fudan University,Shanghai,200031,China;[3]Wenzhou Institute of Shanghai University,Wenzhou,325000,China;[4]Shanghai Frontier Science Center of Optogenetic Techniques for Cell Metabolism,Shanghai,200237,China

年份：2023

期号：11

起止页码：1

中文期刊名：Bioactive Materials

外文期刊名：生物活性材料（英文）

收录：Scopus;CSCD:【CSCD2023_2024】；PubMed;

基金：the National Natural Science Foundation of China(31922041,11932012,32171341);National key research and development program(2021YFB3800800),the 111 project(B14018);the Science and Technology Innovation Project and Excellent Academic Leader Project of Shanghai Science and Technology Committee(21S31901500,21XD1421100);the Scientific and Innovative Action Plan of Shanghai(No.19441900600);the Natural Science Foundation of Shanghai(No.19ZR1408300);the China Postdoctoral Science Foundation(D100-5R-22114);the Shanghai Sailing Program(23YF1409700)are acknowledged.

语种：英文

中文关键词：Injective implant;Suture free;Shape self-adaptive;Intraocular pressure endurance;Foraging behavioral test

摘要：limitation of donor tissue shortage clinically. In addition, suturing-needed transplantation potentially causes postoperative complications. Herein, we design a PEG-Lysozyme injective hydrogel as a suture-free, shape self-adaptive, bioactive implant for corneal stroma defect repair. This implant experiences a sol-gel phase transition via an in situ amidation reaction between 4-arm-PEG-NHS and lysozyme. The physicochemical properties of PEG-Lysozyme can be tuned by the components ratio, which confers the implant mimetic corneal modulus and provides tissue adhesion to endure increased intraocular pressure. In vitro tests prove that the implant is beneficial to Human corneal epithelial cells growth and migration due to the bioactivity of lysozyme. Rabbit lamellar keratoplasty experiment demonstrates that the hydrogel can be filled into defect to form a shape-adaptive implant adhered to native stroma. The implant promotes epithelialization and stroma integrity, recovering the topology of injured cornea to normal. A newly established animal forging behavior test prove a rapid visual restoration of rabbits when use implant in a suture free manner. In general, this work provides a promising preclinical practice by applicating a self-curing, shape self-adaptive and bioactive PEG-Lysozyme implant for suture-free stroma repair.