文献类型：期刊文献

英文题名：High-level expression of a β-mannanase (manB) in Pichia pastoris GS115 for mannose production with Penicillium brevicompactum fermentation pretreatment of soybean meal

作者：Chen, Mianhui[1];Wang, Jingjing[1];Lin, Lin[2,3];Wei, Wei[1];Shen, Yaling[1];Wei, Dongzhi[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Newworld Inst Biotechnol, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Univ Med & Hlth Sci, Shanghai Key Lab Mol Imaging, Shanghai 201318, Peoples R China;[3]Natl Engn Res Ctr Nanotechnol, Res Lab Funct Nanomat, Shanghai 200241, Peoples R China

年份：2021

卷号：44

期号：3

起止页码：549

外文期刊名：BIOPROCESS AND BIOSYSTEMS ENGINEERING

收录：;EI(收录号：20204709501188);WOS:【SCI-EXPANDED(收录号:WOS:000590044800002)】；

语种：英文

外文关键词：beta-Mannanase; Soybean meal; Bio-fermentation pretreated; Mannose; Penicillium brevicompactum

摘要：An endo-1,4-beta-mannanase gene (manB) from a Bacillus pumilus Nsic-2 grown in a stinky tofu emulsion was cloned and expressed in Pichia pastoris GS115. After characterized, the endo-1,4-beta-mannanase (manB) show maximum activity at pH 6.0 and 50 degrees C with LBG as substrate and perform high stability at a range of pH 6-8. After applying for a shake flask fermentation, the specific activity of manB reached 3462 U/mg. To produce mannose, the soybean meal (SBM) was pretreated by biological fermentation for 11 days with Penicillium brevicompactum, and then hydrolyzed by manB. As a result, mannose yield reached 3.58 g per 1 kg SBM which indicated that 0.358% SBM was converted into mannose after hydrolyzation, and mean a total 20% mannan of SBM converting into mannose, while the control group demonstrated only 1.78% conversion. An effective beta-mannanase for the bioconversion of mannan-rich biomasses and an efficient method to produce mannose with soybean meal were introduced.