文献类型：期刊文献

中文题名：粪产碱杆菌青霉素酰化酶在枯草芽孢杆菌中的表达优化

英文题名：Improvement of Culture Conditions to Enhance the Expression of Alcaligenes faecalis Penicillin G Acylase in Bacillus subtilis

作者：史敏龙[1];叶勤[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2003

卷号：29

期号：6

起止页码：575

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;国家哲学社会科学学术期刊数据库;EI(收录号：2004068009626);Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：上海市重点学科项目部分资助

语种：中文

中文关键词：青霉素G酰化酶;枯草杆菌WB600(pMA5);补料分批发酵;葡萄糖流加

外文关键词：penicillin G acylase; Bacillus subtilis WB600 (pMA5); fed-batch culture; glucose feeding

摘要：在5L发酵罐中进行了表达粪产碱杆菌青霉素G酰化酶的重组枯草杆菌WB600(pMA5)的发酵研究。实验表明,发酵液中的葡萄糖浓度和菌体产酶能力密切相关,维持低葡萄糖水平,可以提高枯草杆菌WB600(pMA5)青霉素G酰化酶的合成能力。采用混合碳源进行发酵,在生长阶段流加葡萄糖,并维持低浓度,在发酵12h菌体浓度达到约13.8g/L时停止流加葡萄糖,使菌体利用发酵液中的可溶性淀粉作为碳源,避免葡萄糖的代谢副产物对菌体产酶能力的抑制,青霉素G酰化酶的表达水平从原来的55u/L提高到582u/L。
Cultivations of Bacillus subtilis WB600(pMA5) expressing penicillin G acylase (PGA) were conducted in a 5 L jar fermentor. It was found that glucose exerted great influence on the production of penicillin G acylase, and the maintenance of low glucose levels was favorable to the expression of penicillin G acylase. Mixed carbon sources were used in the fermentation process. During the growth phase glucose was continuously added to maintain low levels so that the metabolic byproducts that repress the expression of penicillin G acylase could be dramatically lessened. At 12 h when the cell concentration reached about 13.8 g/L, the glucose feeding was stopped, and the starch remained in the medium was used as carbon source exclusively. Through this way the inhibition caused by the byproducts of glucose could be lightened, even eliminated completely. Under the improved conditions penicillin G acylase activity was increased from 55 u/L to 582 u/L.