文献类型：期刊文献

英文题名：Separation and Purification of Bitespiramycin by Counter-Current Extraction

作者：Wan, Qianyu[1];Chen, Kui[1];Zhu, Jiawen[1];Wu, Bin[1];Ji, Lijun[1];Wu, Yanyang[1]

机构：[1]E China Univ Sci & Technol, Chem Engn Res Ctr, Shanghai 200237, Peoples R China

年份：2015

卷号：50

期号：17

起止页码：2649

外文期刊名：SEPARATION SCIENCE AND TECHNOLOGY

收录：;EI(收录号：20154601556213);WOS:【SCI-EXPANDED(收录号:WOS:000364327400005)】；

基金：This work was supported by the Science and Technology Commission of Shanghai Municipality (Program No. 10431901500) and was also supported by Tonglian Pharmaceutical Co., Ltd (Shanghai, China).

语种：英文

外文关键词：bitespiramycin; spiramycin; single stage extraction; three-stage counter-current extraction; theoretical model

摘要：To separate spiramycin from the crude sample of bitespiramycin, the optimal extraction conditions in single stage extraction had been obtained as follows: the temperature was 30-40 degrees C, the concentration of phosphate solution was 0.3-0.4 wt.%, and the volumetric ratio of ester/aqueous was 2-3. A three-stage counter-current extraction technique had been utilized to improve extraction efficiency. The removal rate of spiramycin and the retention rate of bitespiramycin were as high as 79.14% and 92.41%, respectively. A theoretical model of three-stage counter-current extraction was introduced to calculate the results of the extraction and fitted the experimental data with satisfactory accuracy.