文献类型：期刊文献

英文题名：RQ feedback control for simultaneous improvement of GSH yield and GSH content in Saccharomyces cerevisiae T65

作者：Xiong, Zhi-Qiang[1,2];Guo, Mei-Jin[1];Guo, Yuan-Xin[1];Chu, Ju[1];Zhuang, Ying-Ping[1];Wang, Nam Sun[2];Zhang, Si-Liang[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Univ Maryland, Dept Chem & Biomol Engn, College Pk, MD 20742 USA

年份：2010

卷号：46

期号：7

起止页码：598

外文期刊名：ENZYME AND MICROBIAL TECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000278612700010)】；

基金：We are grateful to Zhengshen Biotechnical Co., Ltd. (Gansu Province, PR China) for financial support and its industrial strain for GSH production. This work was supported by High-tech Research Plan of China (863) (2007AA100601) and by the Shanghai Leading Academic Discipline Project (B505).

语种：英文

外文关键词：Reduced glutathione; Saccharomyces cerevisiae; Respiratory quotient; Feedback control; Fed-batch phase; Oxygen uptake rate; Carbon dioxide evolution rate

摘要：In reduced glutathione (GSH) production by high-cell-density fed-batch cultivation of Saccharomyces cerevisiae T65, a respiratory quotient (RQ) feedback control strategy was applied to determine glucose feeding rate (F) based anon-line off-gas monitoring in a 15-l stirred bioreactor. Glucose feed was manipulated by a classical proportional-integral-derivative (PID) controller to control RQ at its set-point. RQ controlled at 0.65 resulted in the highest GSH productivity (46.9 mg/l/h) and cell productivity (3.5 g/l/h) as well as improved GSH yield and intracellular GSH content. RQ feedback control achieved yeast dry weight (126 g/l), GSH yield (2.1 g/l), GSH content (1.67%) and GSH productivity (55.3 mg/l/h). which improved by 11.5%, 75%, 57.5% and 82.5%, respectively, compared with conventional ethanol feedback control in the GSH industry. Moreover. RQ feedback control reduced ethanol (byproduct) level to below 0.3 g/l. Advantages of RQ feedback control over the conventional ethanol feedback control in the GSH industry include lower byproduct concentration, shorter cultivation period, higher GSH content, and higher GSH yield. Hence, feedback control based on RQ is a promising method that poses as a simple and efficient alternative to conventional feed control techniques presently practiced in the GSH industry. (C) 2010 Published by Elsevier Inc.