文献类型：期刊文献

英文题名：Enhancement of cell growth and glycolic acid production by overexpression of membrane-bound alcohol dehydrogenase in Gluconobacter oxydans DSM 2003

作者：Zhang, Huan[1];Shi, Lulu[1];Mao, Xinlei[1];Lin, Jinping[1];Wei, Dongzhi[1]

机构：[1]East China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2016

卷号：237

起止页码：18

外文期刊名：JOURNAL OF BIOTECHNOLOGY

收录：;EI(收录号：20163702809116);WOS:【SCI-EXPANDED(收录号:WOS:000385520200003)】；

基金：This work was supported by the National Key Basic Research Development Program of China ("973" Program; No. 2012CB721003), the Shanghai Natural Science Foundation (No. 15ZR1408600), the National Major Science and Technology Projects of China (No. 2012ZX09304009), and the National High Technology Research and Development Program of China ("863" Program; No. 2012AA022201C).

语种：英文

外文关键词：Gluconobacter oxydans; Alcohol dehydrogenase; Respiratory chain; Glycolic acid

摘要：Membrane-bound alcohol dehydrogenase (mADH) was overexpressed in Gluconobacter oxydans DSM 2003, and the effects on cell growth and glycolic acid production were investigated. The transcription levels of two terminal ubiquinol oxidases (bo(3) and bd) in the respiratory chain of the engineered strain G. oxydans-adhABS were up-regulated by 13.4- and 3.8-fold, respectively, which effectively enhanced the oxygen uptake rate, resulting in higher resistance to acid. The cell biomass of G. oxydans-adhABS could increase by 26%-33% when cultivated in a 7L bioreactor. The activities of other major membrane-bound dehydrogenases were also increased to some extent, particularly membrane-bound aldehyde dehydrogenase (mALDH), which is involved in the catalytic oxidation of aldehydes to the corresponding acids and was 1.26-fold higher. Relying on the advantages of the above, G. oxydans-adhABS could produce 73.3 gl(-1)glycolic acid after 45 h of bioconversion with resting cells, with a molar yield 93.5% and a space-time yield of 1.63 gl(-1)h(-1). Glycolic acid production could be further improved by fed-batch fermentation. After 45 h of culture, 113.8 gl(-1) glycolic acid was accumulated, with a molar yield of 92.9% and a space-time yield of 2.53 gl(-1)h(-1), which is the highest reported glycolic acid yield to date. (C) 2016 Elsevier B.V. All rights reserved.