文献类型：期刊文献

英文题名：Mitochondria-targeted fluorescent probe for rapid detection of thiols and its application in bioimaging

作者：Zhu, Yuedong[1,2];Pan, Haiting[2];Song, Yanyan[1,2];Jing, Chao[1,2];Gan, Jia-An[1,2];Zhang, Junji[1]

机构：[1]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Ctr Materio Biol & Dynam Chem, Sch Chem & Mol Engn,Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Ctr Materio Biol & Dynam Chem, Sch Chem & Mol Engn,Joint Int Res Lab Precis Chem, Shanghai 200237, Peoples R China

年份：2021

卷号：191

外文期刊名：DYES AND PIGMENTS

收录：;EI(收录号：20211610235834);WOS:【SCI-EXPANDED(收录号:WOS:000652612300003)】；

基金：The project was supported by NSFC (21878086) , the Shanghai RisingStar Program (19QA1402500 to J. Z.) , Shanghai Sailing Program (18YF1405700 to C. J.) and the Fundamental Research Funds for the Central Universities (222201717003) . The authors acknowledge also acknowledge the Shanghai Municipal Science and Technology Major Project (Grant No.2018SHZDZX03) and the international cooperation program of Shanghai Science and Technology Committee (17520750100) .

语种：英文

外文关键词：Cyanine dyes; Biothiols; Mitochondria-targetable; Oxidative stress; PET

摘要：Biothiols are crucial reactive sulfur species in living cells, playing an essential role in maintaining the normal function of organisms. Effective detection of biothiols promotes the exploration of metabolic pathways and physiological functions of organisms. In this paper, a cyanine-based, mitochondria-targetable fluorescent probe, Cy-DNBS, was designed and synthesized. This probe uses a lipophilic cation unit as mitochondrial targeting site and DNBS group as the fluorescence quencher as well as the recognition site. The probe shows a distinct fluorescence emission at 604 nm upon rapid interaction with intracellular biothiols, owing to the removal of the 2,4dinitrobenzene-sulfonyl (DNBS) group and the blockage of PET process. Moreover, the probe can not only image endogenous thiols, but also efficiently target mitochondria and evaluate thiol fluctuations under mitochondrial oxidative stress induced by H2O2. Collectively, the new low cytotoxic probe can serve as a promising tool for monitoring cellular thiols and further exploring elevant disease processes.