文献类型：期刊文献

英文题名：Light-induced protein degradation in human-derived cells

作者：Sun, Wansheng[1];Zhang, Wenyao[1,2];Zhang, Chao[1];Mao, Miaowei[1,2];Zhao, Yuzheng[1,2];Chen, Xianjun[1,2];Yang, Yi[1,2]

机构：[1]East China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Synthet Biol & Biotechnol Lab, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China

年份：2017

卷号：487

期号：2

起止页码：241

外文期刊名：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000401683700009)】；

基金：This work was supported by NSFC (91313301, 31225008, 31170815 and 31470833 to Y.Y., 31600688 to X. Chen), Specialized Research Fund for the Doctoral Program of Higher Education (20100074110010 to Y.Y), China Postdoctoral Science Foundation (2016M59027 to X. Chen), Shanghai Science and Technology Commission (12JC1402900 and 11DZ2260600 to Y.Y), Dawn Program of Shanghai Education Commission (11SG31 to Y.Y), State Key Laboratory of Bioreactor Engineering, the 111 Project (B07023 to Y.Y) and the Fundamental Research Funds for the Central Universities (to Y.Y).

语种：英文

外文关键词：Light; Protein degradation; Light-switchable degron

摘要：Controlling protein degradation can be a valuable tool for posttranslational regulation of protein abundance to study complex biological systems. In the present study, we designed a light-switchable degron consisting of a light oxygen voltage (LOV) domain of Avena sativa phototropin I (AsLOV2) and a C-terminal degron. Our results showed that the light-switchable degron could be used for rapid and specific induction of protein degradation in HEK293 cells by light in a proteasome-dependent manner. Further studies showed that the light-switchable degron could also be utilized to mediate the degradation of secreted Gaussia princeps luciferase (GLuc), demonstrating the adaptability of the light-switchable degron in different types of protein. We suggest that the light-switchable degron offers a robust tool to control protein levels and may serves as a new and significant method for gene- and cell-based therapies. (C) 2017 Elsevier Inc. All rights reserved.