文献类型：期刊文献

中文题名：螺旋霉素产生菌抗噬菌体菌株的选育

英文题名：Screening of Streptomyces Spiramyceticus for Phage Resistant Strain

作者：叶蕊芳[1];郑黎[1];谢志梅[1];杨漓[1];唐光燕[1]

机构：[1]华东理工大学生物工程系

年份：1997

卷号：23

期号：5

起止页码：545

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;国家哲学社会科学学术期刊数据库;EI(收录号：1998034140004);Scopus;北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：螺旋霉素;抗噬菌体菌株;选育;发酵;摇瓶

外文关键词：streptomycin; Streptomycin spiramyceticus; phage; phage resistant strain; screening

摘要：用Ｃｏ６０处理螺旋霉素产生菌ＰＳ，获得了一株抗噬菌体且摇瓶发酵单位比原菌株稍高的突变株Ｒ１０，继而对Ｒ１０进行理性化筛选，在加有２－脱氧葡萄糖的分离培养基上分离到一株抗噬菌体稳定、摇瓶发酵单位又比Ｒ１０高２０％的新突变株，且其摇瓶发酵周期缩短１２ｈ、能耐受更高浓度的自身代谢产物——螺旋霉素。
A high spiramycin yielding and phage resisting mutant was obtained by way of mutagenesis. The starting strain Streptomycin spiramyceticus PS was first treated with Co irradiation, the phage resisting mutant R10 isolated with a slightly higher yield. Then from strain R10 through rational screening, the strain T 8 was isolated on a plating medium, containing 2 deoxy glucose. T 8 has the stable phage resisting feature with a 20% increase in yield and 12h shortening in fermentation time when cultivated in shaking flask as compared with R10.