文献类型：期刊文献

英文题名：Effects of flow field on the metabolic characteristics of Streptomyces lincolnensis in the industrial fermentation of lincomycin

作者：Li, Xiao[1,2]; Zhang, Jiang[1]; Tan, Ya-Li[1]; Li, Zhi-Hong[2]; Yu, Xue-Feng[2]; Xia, Jian-Ye[3]; Chu, Ju[3]; Ge, You-Qun[4]

机构：[1] College of Chemistry and Biology Science, China Three Gorges University, Yichang 443003, China; [2] Hubei Province Key Lab. of Yeast Function, Angel Yeast Co., Ltd, Yichang 443003, China; [3] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; [4] Jiangxi GuoYao Pharmaceutical Limited Liability Company, Nanchang 330050, China

年份：2013

卷号：115

期号：1

起止页码：27

外文期刊名：Journal of Bioscience and Bioengineering

收录：EI(收录号：20124815725734)

语种：英文

外文关键词：Axial flow - Bacteria - Dissolved oxygen - Fermentation - Fermenters - Flow fields - Impellers - Kinetic energy - Kinetics - Mass transfer - Metabolism - Radial flow - Shear flow - Volumetric analysis

摘要：In this work, the flow field in the existing fermentor with radial-flow impellers (C1) was studied using the computational fluid dynamics (CFD) software package Fluent, then the fermentor with radial-axial flow impellers (C2) was constructed and was compared with the C1 fermentor by CFD and experimental research. The simulation results revealed that the flow field in C2 fermentor had characteristics such as higher turbulent kinetic energy, gas holdup and shear rates. Metabolic variables of Streptomyces lincolnensis in the two fermentors such as carbon and nitrogen source consumption rates, specific growth rates (μ), hyphae morphologies, and lincomycin productivities were further explored. The correlation analysis between the experimental measurements and the simulation results indicated that the hyphae clustering and dry cell weight (DCW) decreasing at production stage were eliminated in C2 fermentor, which had higher gas volumetric mass transfer coefficient (KLa), dissolved oxygen (DO) concentration and consumption rates of nutrient materials. When C2 was employed in the fermentor, the specific growth rate of S. lincolnensis at growth stage was higher, and the maintenance metabolism together with secondary metabolism at production stage was kept at higher levels. As a result, the yield of lincomycin was achieved 7039 μg ml-1 when the 60 m3 industrial fermentor was equipped with C2, which was increased by 46% compared to that obtained in the C1 fermentor. ? 2012 The Society for Biotechnology, Japan.