文献类型：期刊文献

英文题名：Click reaction-based single-particle dark-field microscopy: A visual platform for the highly sensitive and selective detection of pyrophosphatase

作者：He, Cai-Hong[1];Chen, Hua-Ying[1];Jiang, Lei[1];Jin, Zi-Yue[1];Qian, Ruo-Can[1];Chen, Bin-Bin[1,2];Li, Da -Wei[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Frontiers Sci Ctr Materiobiol & Dynam Chem, Shanghai Key Lab Funct Mat Chem,Joint Int Lab Prec, Shanghai 200237, Peoples R China;[2]Chinese Univ Hong Kong, Shenzhen Inst Aggregate Sci & Technol, Sch Sci & Engn, Shenzhen CUHK Shenzhen, 2001 Longxiang Blvd, Shenzhen 518172, Guangdong, Peoples R China

年份：2023

卷号：396

外文期刊名：SENSORS AND ACTUATORS B-CHEMICAL

收录：;EI(收录号：20233714710350);WOS:【SCI-EXPANDED(收录号:WOS:001075496200001)】；

基金：This work was financially supported by the National Natural Science Foundation of China (Grant No. 21974046, 22176058, and 21977031) , Science and Technology Commission of Shanghai Municipality (Grant No. 19520744000, 22ZR1416800, and 23ZR1416100) , the Program of Introducing Talents of Discipline to Universities (B16017) , and the Fundamental Research Funds for the Central Universities (222201717003) . The authors thank Research Center of Analysis and Test of East China University of Science and Technology for the help on the characterization.

语种：英文

外文关键词：Pyrophosphatase; Gold nanoparticle; Click reaction; Localized surface plasmon resonance; Dark-field microscopy

摘要：Inorganic pyrophosphatase (PPase) plays significant roles in diverse pathophysiological processes, therefore, developing a reliable method to measure PPase activity is crucial for the early diagnosis of the related diseases. In this work, a novel platform with single-particle dark-field microscopy (DFM), consisting of core gold nano particles (AuNPs), satellite AuNPs, pyrophosphate ions (PPi), copper ions (Cu2+), and sodium ascorbate (NaAsc), is designed for the selective and sensitive analysis of PPase according to a click reaction. After the introduction of PPase, the coordination between PPi and Cu2+ can be destroyed by catalyzing the hydrolysis of PPi to release free Cu2+ which can be reduced by NaAsc to produce cuprous ions (Cu+). Then the formed Cu+ promotes the click reaction between azide-modified core AuNPs and alkynyl-modified satellite AuNPs to cause the significant redshift of the localized surface plasmon resonance (LSPR) spectra of core AuNPs. Thus, PPase can be ultra sensitively detected with a low detection limit of 0.003 U/L in a wide linear range from 0.01 to 1 U/L. This click reaction-based DFM technique can also be used to assess PPase inhibition, which suggests that it has a lot of potential for both the screening of PPase inhibitors and the diagnosis of PPase-related disorders.