文献类型：期刊文献

英文题名：Domestication of Tartary Buckwheat Shaped a Regulatory Module for Seedling Salt Tolerance by Targeting the Magnesium Transporter Gene FtMGT2

作者：Lu, Xiang[1];He, Yuqi[1];Weng, Wenfeng[1,2];Liu, Zebin[3];Gao, Yuanfen[1];Shi, Yaliang[1];Li, Wei[1];Lai, Dili[1];Zhao, Mengyu[1];Jha, Rintu[1];Zhao, Hui[1,4];Li, Guangsheng[1];Guan, Chaonan[1];Shao, Shuai[5];Ruan, Jingjun[6];Woo, Sun Hee[7];Ouyang, Yinan[1];Quinet, Muriel[8];Georgiev, Milen I.[9,10];Fernie, Alisdair R.[10,11];Hou, Congcong[3];Zhang, Kaixuan[1];Liu, Xu[1];Zhou, Meiliang[1]

机构：[1]Chinese Acad Agr Sci, Inst Crop Sci, State Key Lab Crop Gene Resources & Breeding, Beijing 100081, Peoples R China;[2]Zunyi Normal Univ, Coll Biol & Agr, Zunyi 563099, Peoples R China;[3]Capital Normal Univ, Coll Life Sci, Beijing 100048, Peoples R China;[4]Sichuan Agr Univ, Coll Agr, Chengdu 611130, Peoples R China;[5]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[6]Guizhou Univ, Coll Agr, Guiyang 550025, Peoples R China;[7]Chungbuk Natl Univ, Dept Agron, Cheongju 28644, South Korea;[8]Univ Catholic Louvain, Earth & Life Inst Agron, Grp Rech Physiol Vegetale, Croix Sud 4-5,Boite L7 07 13, B-1348 Louvain La Neuve, Belgium;[9]Bulgarian Acad Sci, Inst Microbiol, Lab Metabol, Plovdiv 4000, Bulgaria;[10]Ctr Plant Syst Biol & Biotechnol, Plovdiv 4000, Bulgaria;[11]Max Planck Inst Mol Plant Physiol, Dept Root Biol & Symbiosis, D-14476 Potsdam, Germany

年份：2026

卷号：13

期号：8

外文期刊名：ADVANCED SCIENCE

收录：;EI(收录号：20254819593361);WOS:【SCI-EXPANDED(收录号:WOS:001621769100001)】；

基金：The authors thank Prof. Suomin Wang and Prof. Li Wei from Lanzhou University who provided the NMT machine. This research was supported by National Natural Science Foundation of China (32161143005, 32241042, and 32301794), the Youth Innovation Program of Chinese Academy of Agricultural Sciences (Y2022QC02), the Project of Sanya Yazhou Bay Science and Technology City (SCKJ-JYRC-2022-22), and National Key R&D Program of China (2023YFF1002500, 2022YFE0140800, and 2021YFD1200105).

语种：英文

外文关键词：FtMGT2; GWAS; RNA-seq; salt tolerance

摘要：Globally, soil salinization increasingly affects farmland, severely limiting the production of Tartary buckwheat (Fagopyrum tataricum). To identify genetic factors for salt tolerance, we analyzed core Tartary buckwheat accessions and utilized differential expression analysis and genome-wide association studies (GWAS), identifying a key domesticated magnesium transporter protein, FtMGT2. A single nucleotide polymorphism (SNP) genotype (G/A) of a natural variant located in the FtMGT2 promoter was found to be positively associated with the expression of FtMGT2 and salt tolerance variation. Mechanistically, the MADS transcription factor FtAGL16 binds the A variant more strongly. FtAGL16 and the MYB transcription factor FtMYB15L co-regulate FtMGT2 transcription, with FtMYB15L protein stability strictly controlled by the E3 ubiquitin ligase FtBRG1. Intriguingly, under salt stress, FtAGL16 can compete with FtBRG1 for binding to FtMYB15L, stabilizing and accumulating FtMYB15L. This enhances FtMGT2 expression, increasing Mg2+ flux, which in turn enhances the transport activity of the sodium (Na+) transporter FtHKT1. This coordinated action leads to increased Na+ efflux and enhanced salt resistance. This study thereby establishes both the theoretical basis and practical application for targeted molecular breeding to enhance plant salt tolerance.