文献类型：期刊文献

英文题名：Targeted Heating of Enzyme Systems Based on Photothermal Materials

作者：Wang, Xuerui[1];Ou, Gang[2];Zhou, Ke[3,4];Wang, Xiangqing[2];Wang, Licheng[1];Zhang, Xiaoyan[5];Feng, Yi[1];Bai, Yunpeng[5];Wu, Hui[2];Xu, Zhiping[3,4];Ge, Jun[1]

机构：[1]Tsinghua Univ, Dept Chem Engn, Minist Educ, Key Lab Ind Biocatalysis, Beijing 100084, Peoples R China;[2]Tsinghua Univ, Sch Mat Sci & Engn, State Key Lab New Ceram & Fine Proc, Beijing 100084, Peoples R China;[3]Tsinghua Univ, Dept Engn Mech, Appl Mech Lab, Beijing 100084, Peoples R China;[4]Tsinghua Univ, Ctr Nano & Micro Mech, Beijing 100084, Peoples R China;[5]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2019

卷号：20

期号：19

起止页码：2467

外文期刊名：CHEMBIOCHEM

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000483090100001)】；

基金：This work was supported by the National Key Research and Development Plan of China (2016YFA0204300), the National Natural Science Foundation of China (51573085, 21622603, 21878174, 21505044, 21506055), and the Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：biosensors; enzymes; magnetic properties; nanoparticles; photochemistry

摘要：This study demonstrates that the enzymatic reaction rate can be increased significantly by targeted heating of the microenvironment around the enzyme, while maintaining the reaction system at environmental temperature. Enzyme molecules are covalently attached to the surface of Fe3O4@reduced graphite oxide (rGO). Under visible-light irradiation, the reaction rate catalyzed by the enzyme-Fe3O4@rGO system is clearly enhanced relative to that of the free enzyme and a mixture of free enzyme and Fe3O4@rGO. This local heating mechanism contributes to promotion of the enzymatic reactions of the targeted heating of the enzyme (THE) system, which has been validated by using different enzymes, including lipase, glucose oxidase, and organophosphorus hydrolase. These results indicate that targeted heating of the catalytic centers has the same effect on speeding up reactions as that of traditional heating methods, which treat the whole reaction system. As an example, it is shown that the THE system promotes the sensitivity of an enzyme screen-printed electrode by 14 times at room temperature, which implies that the THE system can be advantageous in improving enzyme efficiency, especially if heating the entire system is impossible or could lead to degradation of substrates or damage of components, such as in vitro bioanalysis of frangible molecules or in vivo diagnosis.